Molecular Characterization of Alkaline Phosphatase and PLAP in the Germinal Epithelium of Pb and Se+Zn Treated Adult Sprague Dawley Rats
|Falana BA1,2, Ogundele OM3*, Duru FI2, Oshinubi AA2 and Falode DT4|
|1Department of Anatomy, College of Medicine and Health Sci., Osun State University, Oshogbo, Nigeria|
|2Department of Anatomy, College of Medicine and Health Sciences, University of Lagos, Lagos Sate, Nigeria|
|3Department of Anatomy, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Nigeria|
|4Department of Nursing, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Nigeria|
|*Corresponding Author :||Ogundele OM
Department of Anatomy, College of Medicine and Health Sciences
Afe Babalola University, Ado-Ekiti, Nigeria
E-mail: [email protected]
|Received September 11, 2013; Accepted September 23, 2013; Published September 26, 2013|
|Citation: Falana BA, Ogundele OM, Duru FI, Oshinubi AA, Falode DT (2013) Molecular Characterization of Alkaline Phosphatase and PLAP in the Germinal Epithelium of Pb and Se+Zn Treated Adult Sprague Dawley Rats. Cell Dev Biol 2:122. doi:10.4172/2168-9296.1000122|
|Copyright: © 2013 Falana BA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Aim: The role of alkaline phosphatase in cell metabolism and regulation is essential and cuts across cell proliferation, division, cell death, membrane transport and DNA cleavage. This study addresses the role and importance of alkaline phosphates (ALP) and Placenta Alkaline phosphatase (PLAP) in the cells of germinal epithelium of male Rat testes following induced lead (Pb) toxicity and rejuvenation by Selenium (Se) and Zinc (Zn) treatment.
Method: Adult Sprague-Dawley Rats (males) were divided into 4 groups of 15 animals each and were treated as thus; Group 1: Normal saline and serves as the control, Group 2: 100 mg/Kg BW of Pb only, Group 3: 100 mg/Kg BW of Pb and 2.25 mg/Kg each of Se and Zn and group 4 received 2.25 mg.Kg each of Se and Zn only. The duration of treatment was 56 days following which the animals were sacrificed by the 57th day and testes fixed in Bouin’s fluid.
Result: Pb induced toxicity could be apoptotic involving ROS activation of NO-dependent apoptotic pathway or necrotic involving a wide range damage by ROS induced lipid peroxidation. Selenium and zinc treatment ameliorated the damage induced by lead toxicity in the germinal epithelium.
Conclusion: ALP is involved in intrinsic regulation of DNA cleavage in apoptosis by functioning similar to endonucleases and also regulates membrane transport in perioxidated biomembranes. PLAP expression is minimal as tumorgenesis was not recorded, although Pb treatment showed signs of epithelium of endothelial cancer signalling, which was ameliorated by Se+Zn treatment.