alexa Molecular Characterization of Bacterial Phylogenetic an
ISSN:2157-7463

Journal of Petroleum & Environmental Biotechnology
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Research Article

Molecular Characterization of Bacterial Phylogenetic and Functional Groups at Terrebonne Bay along the Coastline of the Gulf of Mexico

Ola A Olapade*
Department of Biology and the Center for Sustainability and the Environment, Albion College, 611 East Porter Street, Albion, MI 49224, USA
Corresponding Author : Ola A Olapade
Department of Biology and the Center for Sustainability and the Environment
Albion College, 611 East Porter Street
Albion, MI 49224, USA
Tel: 517-629-0296
Fax: 517-629-0264
E-mail: [email protected]
Received May 01, 2013; Accepted June 20, 2013; Published June 27, 2013
Citation: Olapade OA (2013) Molecular Characterization of Bacterial Phylogenetic and Functional Groups at Terrebonne Bay along the Coastline of the Gulf of Mexico. J Phylogenetics Evol Biol 4:144. doi:10.4172/2157-7463.1000144
Copyright: © 2013 Olapade OA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

The detection and quantification of bacterial phylogenetic and functional groups as well as community diversity at the site of the Deepwater Horizon oil spill in Terrebonne Bay along the Gulf of Mexico were carried out using nucleic acid staining, Fluorescence in situ Hybridization (FISH) and 16S rRNA gene cloning and sequencing approaches. Results from the 16S rRNA gene clone library analysis revealed high occurrences of bacterial members belonging to the Cyanobacteria (28%), β- Proteobacteria (21%), Bacteroidetes (17%), Actinobacteria (12%) and the α- Proteobacteria (10%). Particularly, bacterial members identified within the clone library as belonging to the β- Proteobacteria subclass were mostly hydrocarbon degraders, including Methylibium petroleiphilum, Burkholderia cepacia, Hydrogenophaga taeniospiralis and Methylobacillus flagellates. Simultaneous analyses of both planktonic and benthic bacterial communities by FISH revealed the numerical dominance of members of the type I Methanotrophic Bacteria (MB) over the type II populations. The results from the study clearly reveal a shift in the bacterial community structure and composition in response to the tragic methane and crude oil discharges from the Deepwater Horizon rig along the Gulf of Mexico.

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