alexa Molecular Characterization of Peckia (Pattonella) inter
ISSN: 2157-7145

Journal of Forensic Research
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Research Article

Molecular Characterization of Peckia (Pattonella) intermutans (Walker, 1861) (Diptera: Sarcophagidae) based on the Partial Sequences of the Mitochondrial Cytochrome Oxidase I Gene

Jandui A Amorim1, Carina M Souza2 and Patricia J Thyssen3*

1Federal Institute of Education, Science and Technology of São Paulo, State of São Paulo, Brazil

2State University of Campinas, UNICAMP, Campinas, State of São Paulo, Brazil 13083-970

3Federal University of Pelotas, UFPel, Pelotas, State of Rio Grande do Sul, Brazil 96010-900

*Corresponding Author:
Patricia J Thyssen
Federal University of Pelotas, UFPel
Pelotas, State of Rio Grande do Sul, Brazil 96010-900
Tel: 55 19 99707564
E-mail: [email protected]

Received date: April 11, 2014; Accepted date: May 14, 2014; Published date: May 19, 2014

Citation: Amorim JA, Souza CM, Thyssen PJ (2014) Molecular Characterization of Peckia (Pattonella) intermutans (Walker, 1861) (Diptera: Sarcophagidae) based on the Partial Sequences of the Mitochondrial Cytochrome Oxidase I Gene. J Forensic Res 5:227 doi: 10.4172/2157-7145.1000227

Copyright: © 2014 Amorim JA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

We analyzed nucleotide sequences that constitute a part of the mitochondrial cytochrome oxidase subunit I (COI) gene from individuals of Peckia (Pattonella) intermutans (Walker, 1861) (Diptera: Sarcophagidae) of different populations from the States of São Paulo and Bahia, Brazil. Indices of genetic variability were calculated and phylogenetic tests (Maximum Parsimony) were applied. The presence of at least five haplotypes among sampled individuals was observed. Pairwise sequence differences within P. (P.) intermutans haplotypes were lower than the pairwise sequence differences between the haplotypes and outgroup species. This finding, in addition to the phylogenetic analyzis performed in this study, validates the use of molecular tools for distinguishing species of P. (P.) intermutans from other sarcophagids already molecularly characterized in Brazil so far. The greatest number of haplotypes (n = 3), genetic diversity (π = 0.01), and nucleotidic differences (k = 2.38) were found from specimens in Ubatuba, reflecting the low rate of genetic flow in this population compared to those from other locations; this was probably influenced by the local ecotope, i.e., the tropical rainforest called “Mata Atlântica”. At collection areas within the rainforest, the vegetal cover is well preserved and this could significantly influence evolutive factors such as population size and selection towards genetic drift, supporting variability maintenance. Further investigations should be conducted for a better understanding of this finding and for expanding knowledge about the close relationships among P. (P.) intermutans and other sarcophagids of forensic importance.

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