Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing
|Hirendra nath Banerjee*, Monique Gramby and Zack Hawkins|
|Department of Biological Sciences, Elizabethcity State University under, The University of North Carolina, Elizabethcity, NC-27909, USA|
|Corresponding Author :||Dr. Hirendra nath Banerjee
Biological Sciences Department
Elizabeth City State University
University of North Carolina
1704 Weeksville Road
Elizabethcity, NC-27909, USA
E-mail: [email protected]
|Received August 23, 2011; Accepted October 17, 2011; Published October 19, 2011|
|Citation: Banerjee HN, Gramby M, Hawkins Z (2011) Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing. J Bioprocess Biotechniq 1:105e. doi: 10.4172/2155-9821.1000105e|
|Copyright: © 2011 Banerjee HN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Aim: Rapid detection of H. pylori strains by PCR-Sequencing.
Methods: 16S rDNA amplification by PCR from template genomic DNA,confirmation of amplicon size by agarose gel electrophoresis ,sequencing of amplicons by automated sequencer,analysis of sequences by NCBI -BLAST software.
Results: The PCR -Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be of H. pylori strain#26695.
Conclusion: The pathogenicity of H. pylori depends on the strain of the bacteria, PCR-Sequencing and analysis of the sequence data by BLAST can be a very quick and useful diagnostic method of the pathogen.