alexa Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing | OMICS International | Abstract
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
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Editorial

Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing

Hirendra nath Banerjee*, Monique Gramby and Zack Hawkins
Department of Biological Sciences, Elizabethcity State University under, The University of North Carolina, Elizabethcity, NC-27909, USA
Corresponding Author : Dr. Hirendra nath Banerjee
Associate Professor
Biological Sciences Department
Elizabeth City State University
University of North Carolina
1704 Weeksville Road
Elizabethcity, NC-27909, USA
E-mail: [email protected]
Received August 23, 2011; Accepted October 17, 2011; Published October 19, 2011
Citation: Banerjee HN, Gramby M, Hawkins Z (2011) Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing. J Bioprocess Biotechniq 1:105e. doi: 10.4172/2155-9821.1000105e
Copyright: © 2011 Banerjee HN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Aim: Rapid detection of H. pylori strains by PCR-Sequencing.
Methods: 16S rDNA amplification by PCR from template genomic DNA,confirmation of amplicon size by agarose gel electrophoresis ,sequencing of amplicons by automated sequencer,analysis of sequences by NCBI -BLAST software.
Results: The PCR -Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be of H. pylori strain#26695.
Conclusion: The pathogenicity of H. pylori depends on the strain of the bacteria, PCR-Sequencing and analysis of the sequence data by BLAST can be a very quick and useful diagnostic method of the pathogen.

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