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Molecular Forensic Approaches to the Taxonomic Assessment of Bacteria in a Commercial Consortia | OMICS International | Abstract
ISSN: 2157-7145

Journal of Forensic Research
Open Access

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Research Article

Molecular Forensic Approaches to the Taxonomic Assessment of Bacteria in a Commercial Consortia

Luke Masson1*, Thomas A. Edge2, Christine Maynard1, Roland Brousseau1, Nathalie Fortin1, Charles W. Greer1 and Jack T. Trevors3

1Environmental Sector, Biotechnology Research Institute, NRC, 6100 Royalmount Avenue, Montreal, Quebec, H4P 2R2, Canada

2National Water Research Institute, Environment Canada, 867 Lakeshore Rd., Burlington, Ontario, L7R 4A6, Canada

3School of Environmental Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada

*Corresponding Author:
Dr. Luke Masson
Biotechnology Research Institute
National Research Council of Canada
6100 Royalmount Ave, Montreal
Quebec, Canada, H4P 2R2
Tel: 1-514-496-3123
E-mail: [email protected]

Received Date: July 07, 2011; Accepted Date: July 22, 2011; Published Date: July 27, 2011

Citation: Masson L, Edge TA, Maynard C, Brousseau R, Fortin N, et al. (2011) Molecular Forensic Approaches to the Taxonomic Assessment of Bacteria in a Commercial Consortia. J Forensic Res S2:001. doi: 10.4172/2157-7145.S2-001

Copyright: © 2011 Masson L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The characterization of low complexity (only a few species present) bacterial communities or commercial consortia products in terms of microbial composition can require a variety of molecular techniques for supporting forensic investigations. We examined a low complexity commercial consortium productfor water treatment application as a model for a tiered molecular approach to studying microbial communities. PCR amplification of 16S rDNA and cpn60 genes were performed on total genomic DNA extracted from the consortium. First, the PCR amplicons were cloned, sequenced and subjected to both DGGE and RFLP analysis, or they were fluorescently labeled and hybridized to a dual backbone taxonomic DNA microarray. Secondly, total genomic DNA from the commercial consortium was subjected to quantitative PCR to determine the concentration of the different components.

The data showed that the dual backbone DNA microarray is extremely useful as a first step to identify the major members of the consortium, including lot-to-lot variation of the commercial product, as validated by independent analyses. More importantly, the DNA microarray proved to be a useful screening tool to detect unexpected and potentially pathogenic microbes in the commercial product. This tiered approach using a DNA microarray screen can be a useful guide for application of more rapid and targeted molecular tools in forensic investigations of microbial communities.

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