Molecular Identification of a Parasitic Fly (Diptera: Tachinidae) from the Introduced Helicoverpa armigera (Lepidoptera: Noctuidae) in BrazilGuerra WD1, Guerra ALLD2, Ribas LN3, Gonçalves RM4 and Mastrangelo T4*
- *Corresponding Author:
- Thiago Mastrangelo
Center for Molecular Biology and Genetic Engineering
State University of Campinas (UNICAMP)
Av. Candido Rondon 400, 13083-875, Campinas, SP, Brazil
Tel: +55 19 3521 1141
E-mail: [email protected]
Received date: July 13, 2014; Accepted date: June 24, 2014; Published date: June 26, 2014
Citation: Guerra WD, Guerra ALLD, Ribas LN, Gonçalves RM, Mastrangelo T (2014) Molecular Identification of a Parasitic Fly (Diptera: Tachinidae) from the Introduced Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil. Entomol Ornithol Herpetol 3:131. doi: 10.4172/2161-0983.1000131
Copyright: © 2014 Guerra WD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The African cotton bollworm, Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) was detected in Brazil, triggering a major phytosanitary crisis during the 2012/2013 harvest. Despite its recent introduction, larvae were found being parasitized by indigenous dipterans with estimated parasitism rates even higher than 30% at some localities. Two of these flies from the municipality of Campo Verde, Central-East Mato Grosso, were sent to the State University of Campinas to be identified through mitochondrial DNA. Partial sequences of the mitochondrial cytochrome c oxidase subunit I gene were obtained and matched more than 99% with Archytas marmoratus Towsen (Diptera: Tachinidae) known sequences from BOLD database. The pairwise genetic distances (K2P and p-distance) between the two Brazilian specimens and the 14 other tachinid species were all higher than 4%. The findings of this study serve as first evidence that an indigenous species of tachinid exists in Brazil that may be a good biological agent for the exotic H. armigera.