Molecular Methods in Microbiology and their Clinical Application
- Corresponding Author:
- Malhotra S
Department of Microbiology
PGIMER and Dr RML Hospital New Delhi, India
E-mail: drshalinimalh[email protected]
Received Date: March 14, 2014; Accepted Date: November 20, 2014; Published Date: November 27, 2014
Citation: Malhotra S, Sharma S, Bhatia NJK, Kumar P, Hans C (2014) Molecular Methods in Microbiology and their Clinical Application. J Mol Genet Med 8:142. doi:10.4172/1747-0862.1000142
Copyright: © 2014 Malhotra S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Conventional microbiology is an inexpensive but protracted diagnostic method and there are certain limitations associated with it. Molecular methods have been increasingly incorporated in laboratories, particularly for the detection and characterization of isolates and for the diagnosis of diseases due to fastidious, slow growing, nonviable or non-cultivable organisms which cannot be detected by conventional culture techniques. The introduction of these techniques and their automation provides new opportunities for the clinical laboratory to affect patient care. These tests provide timely results which are useful for high-quality patient care at a reasonable cost and are associated with improvement in patients care. The use of amplification techniques such as polymerase chain reaction, real-time polymerase chain reaction or nucleic acid sequence-based amplification for micro-organisms detection, genotyping and quantification. Molecular methods are rapid and highly sensitive and specific. This review will focus on various molecular techniques which are performed in clinical microbiology laboratories and their clinical applications and therefore help in management of infectious diseases.