Molecular Reclassification in Pediatric Osteosarcomas at Surgical Resection is a Potential Helpful Prognostic Marker
- *Corresponding Author:
- Rajiv Raja
Department of Oncology Biomarker Development
Genentech Inc., MS-461a, 640 East Grand Avenue
South San Francisco, CA 94080, USA
Tel: 650 225 3291
E-mail: [email protected]
Received Date: January 27, 2015; Accepted Date: March 28, 2015; Published Date: March 30, 2015
Citation: Desai R, Patel R, Amler L, Koeppen H, McCaffery I, et al. (2015) A Novel Method for Mutation Analysis Using Genomic DNA Obtained from Immunohistochemistry-Stained Sections. J Mol Biomark Diagn 6:228. doi:10.4172/2155-9929.1000228
Copyright: © 2015 Desai R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Introduction: The management of pediatric high grade osteosarcoma is lacking new approaches to classify closely the patients and adapt thereafter the treatment initially or post-operatively. The objective of this study was to estimate the impact of the tumor molecular response comparatively to initial biopsy and to see if this molecular analysis was correlated to the histological response to neoadjuvant chemotherapy and/or prognosis.
Material and methods: 33 patients were included and allelotyping analyses using 23 micro satellites were performed on biopsy’s and tumor’s DNA versus normal blood DNA. Allelic imbalances were detected in all biopsy samples and the number of persistent AIs or not were quantified on tumors after pre-operative chemotherapy.
Results: We identified 4 subgroups with a significant impact on survival. The first group presents a complete disappearance of the AIs and a complete response independently from the histopathologic measure for tumor necrosis. The second group showed a partial response with persistence of some rearrangements after treatment. The third and the last one were characterized by the same molecular profile even more rearrangements, allowing to considered those subgroups as highly resistant osteosarcomas. This molecular re-stratification was associated with a significant impact on survival and provides evidence that this new approach on tumor resection might be a complementary and useful tool combined with histological response assessment.