alexa Molecular Validation of Putative Antimicrobial Peptides for Improved Human Immunodeficiency Virus Diagnostics via HIV Protein p24 | OMICS International | Abstract
ISSN 2155-6113

Journal of AIDS & Clinical Research
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Molecular Validation of Putative Antimicrobial Peptides for Improved Human Immunodeficiency Virus Diagnostics via HIV Protein p24

Monray Edward Williams1, Marius Tincho1, Musa Gabere2, Ashley Uys3, Mervin Meyer1 and Ashley Pretorius1*

1University of the Western Cape, South Africa

2King Abdullah International Medical Research Center, National Guard Health Affairs, Saudi Arabia

3Medical Diagnostech PTY LTD, South Africa

*Corresponding Author:
Ashley Pretorius
Department of Biotechnology, University of the Western Cape
Life Science Building (Room 2.48), Bellville, South Africa
Tel: + 27 (0) 21 959 9727; +27 (0) 82 329 5646
Fax: + 27 (0) 21 959 3505
E-mail: [email protected]

Received March 14, 2016; Accepted April 26, 2016; Published May 03, 2016

Citation: Williams ME, Tincho M, Gabere M, Uys A, Meyer M, et al. (2016) Molecular Validation of Putative Antimicrobial Peptides for Improved Human Immunodeficiency Virus Diagnostics via HIV Protein p24. J AIDS Clin Res 7:571. doi:10.4172/2155-6113.1000571

Copyright: © 2016 Williams ME, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Objective: Various HIV diagnostic systems are available however; the p24 antigen detection assay is preferred due to its ability to decrease the window period for HIV detection. The p24 antigen assay has been associated with low sensitivity. Antimicrobial Peptides (AMPs) display huge potential in development of more effective diagnostics tools. The aim of this study was to design derivative AMPs from AMPs identified previously, using an in silico approach, as templates, with higher predicted binding affinity for the p24 protein and implement the best suited AMPs in a Point-Of- Care (POC) device for detection of HIV-1 and HIV-2.

Method: This study firstly used an in silico approach to identify derivative AMPs which bind the N-terminal domain of the p24 antigen with increased predicted binding affinity. The in silico approach used the parental AMPs as templates for generation of the derivative AMPs through site-directed mutagenesis, 3-D structure prediction and docking studies. Secondly, the binding between the synthetically synthesized AMPs and the HIV antigen p24 was validated by an in house Lateral Flow Device (LFD) “on/off” binding experiment. The most promising AMPs were conjugated to gold nanoparticles (AuNPs) and implemented in a LFD to detect HIV-1 and HIV-2 using patient sera as well as a panel of international HIV standards (panel C10).

Result: With conjugation of AMPs to gold nanoparticles, parent AMP 1 and derivative AMP 1.1 used in combination most sensitively detected the recombinant protein p24 and was therefore selected for development of a LFD prototype for HIV diagnostics. Global HIV-1 (C10) and HIV-2 (C10) standards were successfully detected by the LFD prototype as well as HIV infected serum samples.

Conclusion: A sensitive HIV-1 and HIV-2 LFD prototype was developed which would within 15 minutes provide patients with accurate and sensitive diagnosis of HIV.


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

chemistryjourn[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7