Morphological and Molecular Characterization of Diplozoon kashmirensis; D. aegyptensis and D. guptai Collected from Fishes of Kashmir Valley- India
- Corresponding Author:
- Tanveer A. Sofi
Department of Zoology
University of Kashmir
E-mail: [email protected]
Received: August 18, 2015; Accepted: August 24, 2015; Published: October 27, 2015
Citation: Ahmad F, Fazili KM, Sofi TA, Sheikh BA, Waza AA (2015) Morphological and Molecular Characterization of Diplozoon kashmirensis; D. aegyptensis and D. guptai Collected from Fishes of Kashmir Valley-India. Fish Aquac J 6:147. doi:10.4172/2150-3508.1000147
Copyright: © 2015 Ahmad F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The study reports the results of molecular characterization of the Internal Transcribed Spacer (ITS) of ribosomal DNA of 3 Monogenean species using polymerase chain reaction (PCR), nucleotide sequencing and construction of phylogenetic trees from different fish hosts of Kashmir. The present study shows that the size of the amplified product is 873bp long for D. kashmirensis, 1120bp long in D. aegyptensis and 687bp long in D. guptai revealing that there are intraspecific differences in their base pair lengths. Guanine and Cytocine (G+C) content of three Diplozoon species was found nearly constant for three species i.e., 47% (D. kashmirensis); 47% (D. aegyptensis) and 48% (D. guptai), this GC richness contributes to physical attributes of RNA structures, as there is correlation between GC content and optimal growth temperature. An important observation during the present study has been noticed that Schizothorax niger is infected by all the three species of Diplozoidae; D. kashmirensis; D. aegyptensis and D. guptai, but when all six fishes were collected simultaneously, parasitism by all the parasite species was never observed. Phylogenetic trees Maximum Parsimony (MP), Maximum Likelihood (ML) and Neighbor Joining (NJ) showed that D. kashmirensis and D. aegyptensis share a common host Carassius carassius and S. niger.