Multicellular Spheroid Culture Models: Applications in prostate Cancer Research and TherapeuticsDaisuke Kurioka1, Akimitsu Takagi2, Misao Yoneda3, Yoshifumi Hirokawa3, Taizo Shiraishi3 and Masatoshi Watanabe1*
- *Corresponding Author:
- Dr. Masatoshi Watanabe, MD, PhD
Laboratory for Medical Engineering
Division of Materials Science and Chemical Engineering
Graduate School of Engineering
Yokohama National University, 79-5 Tokiwadai
Hodogayaku, Yokohama, Japan
E-mail: [email protected]
Received date: November 06, 2010; Accepted date: January 26, 2011; Published date: February 04, 2011
Citation: Kurioka D, Takagi A, Yoneda M, Hirokawa Y, Shiraishi T, et al. (2011) Multicellular Spheroid Culture Models: Applications in prostate Cancer Research and Therapeutics. J Cancer Sci Ther 3: 060-065. doi:10.4172/1948-5956.1000060
Copyright: © 2011 Kurioka D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Prostate cancer is one of the most prevalent cancers in men in Western countries, increasing in frequency with age through the most advanced years. Patients with localized prostate cancer are generally treated with radical prostectomy or radiation therapy. However, treatment of more malignant stages of the disease is problematic. Docetaxel-based chemotherapy in men with androgen-independent prostate cancer has been shown to have survival benefits but hormonal manipulation and other chemotherapeutic regimens, especially for androgen-independent lesions, have uncertain value. While research into the complex pathophysiology of advanced prostate cancer has led to identification of mechanisms and target molecules, it nevertheless remains necessary to develop new anticancer drugs. Cell culture models that mimic the structure and features of prostate cancer in vivo are necessary for research on tumor biology and design of novel anticancer therapies. In this context, 3-dimensional cultures of prostate cancer cells, including multicellular spheroid (MCS) cultures, started attracting increasing attention. The present review provides up-to-date information regarding the significance of MCS culture for identification of mechanisms underlying human malignancies, including prostate cancer, and possible targets for prostate cancer therapies.