Multilocus Genotyping of Asian Elephant Ivory: A Case Study in Suspected Wildlife Crime
Sandeep Kumar Gupta*, Ajit Kumar and Parag Nigam
Wildlife Institute of India, Dehra Dun, India
- *Corresponding Author:
- Dr. S K Gupta, Scientist
Wildlife Institute of India, Dehra,
Dun 248 001, India
Tel: +91-135-2640111-15 (ext. 353)
E-mail: [email protected]
Accepted Date: July 20, 2011; Published Date: July 28, 2011
Citation: Gupta SK, Kumar A, Nigam P (2011) Multilocus Genotyping of Asian Elephant Ivory: A Case Study in Suspected Wildlife Crime. J Forensic Res S1:002. doi: 10.4172/2157-7145.S1-002
Copyright: © 2011 Gupta SK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
As a part of wildlife crime investigation, two pieces of Asian elephant (Elephas maximus) ivory were collected by Uttarakhand forest department at different time interval to identify whether they belonged to same individual. The samples were analyzed using multilocus genotype based genetic analysis. Twelve fluorescent labeled microsatellite loci were used to analyze the nuclear DNA that were developed and validated for Asian elephants. Based on DNA typing, both the samples showed same alleles at every tested locus indicating that both the ivory pieces originated from single individual. The study highlights successful extraction and analysis of nuclear DNA employing microsatellite markers from ivory collected from Asian elephant.