alexa Multiplex PCR-based detection of Salmonella typhimurium and Salmonella enteritidis in Specific Pathogen Free (SPF) and Commercial Eggs
ISSN: 2327-5073

Clinical Microbiology: Open Access
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Research Article

Multiplex PCR-based detection of Salmonella typhimurium and Salmonella enteritidis in Specific Pathogen Free (SPF) and Commercial Eggs

Jakeen El Jakee1, Diaa El Din Gad Khelfa2, Monier Mohammed El-Safty3, Ahmed Adel Seida1*, Sherif Marouf1, Jens Hahne4, Zafar Mahmood5 and Sarah Sobhy Nagy3

1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Cairo University, Egypt

2Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Cairo University, Egypt

3Quality Control of SPF Eggs Department in Central Laboratory for Evaluation of Veterinary Biologics (CLEVB) Abassia, Cairo, Egypt

4Institute of Cancer Research, London, England

5Graduate School of Life Sciences, University of Würzburg, Würzburg, Germany

*Corresponding Author:
Ahmed Adel Seida
Microbiology and Immunology Department, Faculty of Veterinary Medicine
Cairo University, Giza, P.O. 12211, Egypt
Tel: 0020237175004
E-mail: [email protected]

Received date: February 23, 2016; Accepted date: March 28, 2016; Published date: April 04, 2016

Citation: El Jakee J, Khelfa DEDG, El-Safty MM, Seida AA, Marouf S, et al. (2016) Multiplex PCR-based detection of Salmonella typhimurium and Salmonella enteritidis in Specific Pathogen Free (SPF) and Commercial Eggs. Clin Microbiol 5:241. doi:10.4172/2327-5073.1000241

Copyright: © 2016 El Jakee J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Salmonella serovars are one of the major bacterial causes of food borne diseases. Eggs are commonly identified as food sources responsible for salmonellosis outbreaks. This study aimed to isolate Salmonella typhimurium and Salmonella enteritidis from 1750 hens’ eggs, and use of multiplex polymerase chain reaction (Multiplex PCR) in the identification of different Salmonella serovars from eggs. The incidence of salmonellae among the Balady eggs yolk was 1.3%, while the incidence was 1.2% among white and brown eggs samples (each). S. typhimurium and S. enteritidis were identified (0.6 and 0.5% respectively). The isolates were confirmed using fliC, sefA genes and gene specific for genus Salmonella. All albumen samples negative for isolation of isolation of salmonellae by culture method were retested by PCR.

From the retested albumen samples 3%, 8.4% and 6% collected from balady, white and brown eggs respectively were positive for Salmonella serovars using Multiplex PCR. No salmonellae could be detected from specific pathogen free (SPF) eggs using both PCR and conventional methods.

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