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ISSN: 2153-0769

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Short Communication

Multiplexed Quantification of Metabolites with MISSILE

Jones DR1*, Wang X2, Shaw T2,3, Cho JH2 and Peng J2-5*

1Department of Biochemistry and Molecular Pharmacology, Langone Medical Center, New York University, NY, 10016, USA

2St. Jude Proteomics Facility, St. Jude Children's Research Hospital, Memphis, TN 38105, USA

3Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA

4Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA

5Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA

*Corresponding Author:
Jones DR
Department of Biochemistry and Molecular Pharmacology
Langone Medical Center
New York University
550 1st Avenue, NY, 10016, USA
Tel: 646-501-2097
E-mail: [email protected]
Peng J
Department of Structural Biology
St. Jude Children's Research Hospital
262 Danny Thomas Place, Memphis, TN 38105, USA
Tel: (901) 595-7499
Fax: (901) 595-3032
E-mail: [email protected]

Received Date: February 06, 2017; Accepted Date: April 06, 2017; Published Date: April 12, 2017

Citation: Jones DR, Wang X, Shaw T, Cho JH, Peng J (2017) Multiplexed Quantification of Metabolites with MISSILE. Metabolomics (Los Angel). 7:189. doi: 10.4172/2153-0769.1000189

Copyright: © 2017 Jones DR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

We tested a strategy for multiplexed (4-plex) quantification of metabolites using the MISSILE identification method with liquid chromatography coupled to tandem mass spectrometry. We applied this methodology to study the metabolic effect of the proteasome inhibitor and chemotherapeutic drug Bortezomib in yeast cells. Using JUMPm software version 1.1 we simultaneously identified and quantified 95 metabolites across four experimental conditions and found that Bortezomib increased the accumulation of dipeptides but decreased the levels of specific lipid molecules (e.g. phosphtidylethanolamines) in a dose-dependent manner. This method combines metabolite identification and quantification, making untargeted metabolomics experiments more informative.

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