alexa NAD (H) Linked Enzyme Catalyzed Reactions using Coupled Enzymes in a Composite Nanoparticle System
ISSN: 2329-6674

Enzyme Engineering
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Research Article

NAD (H) Linked Enzyme Catalyzed Reactions using Coupled Enzymes in a Composite Nanoparticle System

Sujata Sinha*, Vidya Bhat and Subhash Chand
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi Hauz Khas, New Delhi, India
Corresponding Author : Sujata Sinha
Department of Biochemical Engineering and Biotechnology
Indian Institute of Technology, Delhi Hauz Khas
New Delhi-110016, India
Tel: +91-26591004
Fax: +91-1126582282
E-mail: Sinha738@gmail.com
Received November 11, 2013; Accepted December 03, 2013; Published December 06, 2013
Citation: Sinha S, Bhat V, Chand S (2013) NAD (H) Linked Enzyme Catalyzed Reactions using Coupled Enzymes in a Composite Nanoparticle System. Enz Eng 2:117. doi:10.4172/2329-6674.1000117
Copyright: © 2013 Sinha S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

 Different approaches for cofactor recycling/regeneration have been studied and one of them is having regenerating enzyme immobilized on some suitable support. Here, we are reporting the cofactor NAD (H) recycling with the help of free enzymes and enzymes loaded on nanoparticles. Baker’s yeast alcohol dehydrogenase (ADH) and formate dehydrogenase (FDH) from Candida boidinii were immobilized on alumina nanoparticles and applied to catalyze the coupled reactions for production of n-propanol. Cofactor regeneration within the reaction cycle was achieved as a result of collision between enzyme loaded particles and free cofactor. Brownian motion provided effective interactions among the catalytic components, and thus realized a dynamic shuttling of the cofactor between the two enzymes to keep the reaction cycles continuing. Maximum recycle rate of 6650 cycles/hr was obtained and it decreased with increasing cofactor concentration within the reaction system, for free as well as immobilized system.It was concluded that particles attached enzymes could be one of the new biochemical strategy used for cofactor dependent biotransformation.

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