Nanoparticles Effects on Performance of Horseradish Peroxidase BiosensorSaghafi E1 and Farahbakhsh A2*
- *Corresponding Author:
- Farahbakhsh A
Department of Chemical Engineering
Quchan Branch, Islamic Azad University
E-mail: [email protected]
Received Date: January 16, 2013; Accepted Date: May 14, 2013; Published Date: May 16, 2013
Citation:Saghafi E, Farahbakhsh A (2013) Nanoparticles Effects on Performance of Horseradish Peroxidase Biosensor. J Biosens Bioelectron 4:136. doi: 10.4172/2155-6210.1000136
Copyright: © 2013 Saghafi E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The immobilization of horseradish peroxidase (HRP) enzyme causes protections on catalytic activities in
determination of H2O2. The rate of electron transfer from redox enzyme onto the electrode surface is an important topic in bioelectrochemistry that could improve more the function of HRP biosensor by means of increasing electrode conductivity. Referring to this basis, effect of various nanoparticles are evaluated and compared with each other on the operation promotion in this review and Glassy Carbon Electrode (GCE) is utilized as a main foundation on immobilization of nanoparticles and HRP enzyme. Electrodes have been compared by the rate of current transfer in the optimum voltage data in the presence of Carbon-coated Iron Nanoparticles (CIN), Quantum Dots (QDs), Au and Laponite/Chit nanoparticles. Consequently in the same scan rate, HRP/Au/GCE (28 μA) has achieved more current which is about 94% comparing to the base electrode HRP/GCE (1.8 μA).