NAPPA-Based Nanobiosensors for the Detection of Proteins and of Protein-Protein Interactions Relevant to CancerNicola Luigi Bragazzi1,2, Rosanna Spera1,2, Eugenia Pechkova1,2 and Claudio Nicolini1,2,3*
- *Corresponding Author:
- Claudio Nicolini
Nanoworld Institute Fondazione EL.B.A. Nicolini (FEN)
Largo Redaelli 7, Pradalunga, Bergamo 24100, Italy
E-Mail: [email protected]
Received date: November 06, 2013; Accepted date: March 22, 2014; Published date: March 27, 2014
Citation: Bragazzi NL, Spera R, Pechkova E, Nicolini C (2014) NAPPA-Based Nanobiosensors for the Detection of Proteins and of Protein-Protein Interactions Relevant to Cancer. J Carcinog & Mutagen 5:166. doi: 10.4172/2157-2518.1000166
Copyright: © 2014 Bragazzi NL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this manuscript, we report some proofs-of-principle and preliminary applications and results regarding the analytical quantification of protein expression of some genes biologically and clinically related to cancer. Experiments have been carried out coupling Nucleic Acid Programmable Protein Array (NAPPA) with a recently improved nanogravimetric apparatus which exploits the quartz crystal microbalance with frequency (QCM_F) and quartz crystal microbalance with dissipation monitoring (QCM_D) technologies. The selected proteins are BRIP1, JUN and ATF2. Clinically relevant implications for cancer are envisaged and discussed, as well as future perspectives and developments.