Nasal Administration of Glucosyltransferase-I of Streptococcus sobrinus without Adjuvant Induces Protective Immunity
- *Corresponding Author:
- Dr. Masafumi Yamamoto
Department of Microbiology and Immunology
Nihon University School of Dentistry at Matsudo
2-870-1 Sakaecho- Nishi, Matsudo, Chiba 271-8587, Japan
Fax: +81-47-360- 9601
E-mail: [email protected]
Received Date: December 08, 2010; Accepted Date: December 30, 2010; Published Date: December 30, 2010
Citation: Watanabe K, Hashizume T, Kurita-Ochiai T, Akimoto Y, Yamamoto M (2010) Nasal Administration of Glucosyltransferase-I of Streptococcus sobrinus without Adjuvant Induces Protective Immunity. J Vaccines Vaccin 1:109. doi: 10.4172/2157-7560.1000109
Copyright: © 2010 Watanabe K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This study seeks to assess the efficacy of the glucosyltransferase-I (GTF-I) produced by Streptococcus sobrinus as a nasal vaccine for the prevention of dental caries. Nasal immunization of mice with GTF-I elicited significant levels of GTF-I-specific IgG and IgA in serum and mucosal IgA antibodies in saliva. Antibody-forming cell analysis confirmed the antibody titers by detecting high numbers of GTF-I-specific antibody-forming cells in spleen and salivary glands. Coadministration of GTF-I and oligodeoxynucleotides (ODN) containing unmethylated cytosine-phosphate-guanine (CpG) dinucleotides (CpG ODN) as adjuvant further elevated salivary IgA antibody responses; however, the increase in serum antibody levels was slight. GTF-I-specific IgG antibodies from mice given GTF-I alone or GTF-I plus CpG ODN inhibited biofilm formation by S. sobrinus. Finally, the mice given nasal GTF-I showed protection and significant inhibition of dental caries caused by oral infection with S. sobrinus even without the use of CpG ODN. These results suggest that nasal administration of GTF-I could be an important tool for the prevention of dental caries.