Neuroprotective Effect of (-)-Linalool against Sodium Nitroprusside- Induced Cytotoxicity
- *Corresponding Author:
- Geun Hee Seol
Professor, Department of Basic Nursing Science
School of Nursing, Korea University
145 Anam-ro, Seongbuk-gu
Seoul 136-701, Republic of Korea
E-mail: [email protected]
Received date: March 25, 2015; Accepted date: April 16, 2015; Published date: April 18, 2015
Citation: Kim KY, Lee HS, Min SS, Seol GH (2015) Neuroprotective Effect of (-)-Linalool against Sodium Nitroprusside-Induced Cytotoxicity. Med chem 5:178-182. doi:10.4172/2161-0444.1000261
Copyright: © 2015 Kim KY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
(-)-Linalool has various pharmacological effects in humans and animal models, especially in the central nervous system. This study investigated whether (-)-linalool and linalyl acetate, the major components of lavender, can protect SH-SY5Y cells against sodium nitroprusside (SNP)-induced cytotoxicity. Cell viabilityand nitric oxide (NO) production in the presence of SNP and (-)-linalool was assessed by MTT and nitrite assays, and the free radical-scavenging activity of (-)-linalool was assessed by DPPH assay. (-)-Linalool, at low, non-toxic concentrations of 1 μM (p=0.003), 2.5 μM (p=0.001), and 5 μM (p=0.008), significantly enhanced neuronal cell viability in the presence of SNP. The protective effect of (-)-linalool against SNP-induced cytotoxicitywas also confirmed by Hoechst staining. SNP-induced NO production was significantly decreased (p<0.001 each), and antioxidant levels significantly increased (p≤0.001), by 1, 2.5, and 5 μM (-)-linalool. These findings, showing that (-)-linalool protected SH-SY5Y cells against SNP-induced cytotoxicity by decreasing the production of NO, suggested that (-)-linalool has anti-oxidant activity in the central nervous system and may be a potential therapeutic drug in patients with neurodegenerative diseases.