alexa New Method to Restore Fading Papanicolaou Specimen Using an Optical Clearing Agentx | OMICS International | Abstract
ISSN: 2157-7099

Journal of Cytology & Histology
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New Method to Restore Fading Papanicolaou Specimen Using an Optical Clearing Agentx

Kiyotada Washiya1*, Makoto Sakurai1, Saki Furutate1, Estuko Okusawa2, Shigeharu Hatakeyama3, Haruhiko Yoshioka1 and Jun Watanabe1

1Department of Pathologic Analysis, Hirosaki University Graduate School of Health Sciences, Japan

2Department of Pathology, Hachinohe City Hospital, Hachinohe, Japan

3Cytology & Pathology Research Center, Bunkyo-ku, Tokyo 113-0034, Japan

*Corresponding Author:
Kiyotada Washiya
Department of Pathologic Analysis
Hirosaki University Graduate School of Health Sciences
66-1 Honcho, Hirosaki-shi, Aomori 036-8564, Japan
Tel: +81 172 39 5972
E-mail: [email protected]

Received Date: October 23, 2013; Accepted Date: February 17, 2014; Published Date: February 19, 2014

Citation: Washiya K, Sakurai M, Furutate S, Okusawa E, Hatakeyama S (2014) New Method to Restore Fading Papanicolaou Specimen Using an Optical Clearing Agent. J Cytol Histol 5:219. doi:10.4172/2157-7099.1000219

Copyright: © 2014 Washiya K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Cytological specimens are used only once for staining, and cannot be reused after preservation in paraffin blocks. If a technique is developed allowing restaining of precious/rare specimens or those for education, which have been discolored due to long-term preservation, to restore their former states, it will be extremely useful for the reuse of specimens. We performed this study to determine whether discolored specimens can be restored to their former condition by applying an optical clearing agent. For restaining, 131 Papanicolaou (Pap) specimens prepared from 13 to more than 30 years ago were used. Using an optical clearing agent (SCALEVIEW-A2), Pap restaining, endurance tests of the optical clearing agent by repeated use, and immunostaining were performed. After treatment with the optical clearing agent for5-10 minutes, discolored cytological specimens could be stably restained just as the initial Pap specimens. As a result of the immunostaining of specimens treated using this method, both the nucleus (Ki-67) and cell membrane (MOC31) were positively stained. The optical clearing agent did not deteriorate when used repeatedly ten times. Discolored precious cytological specimens could be restored using a optical clearing agent. Specimens processed using this method could also be immunostained, and so this method is extremely useful

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