alexa No Evidence of Interspecific Genetic Exchange by Multi-Locus Microsatellite Typing Between Leishmania Killicki and Leishmania Major in a Mixed Focus of Cutaneous Leishmaniasis in Southeast Tunisia
ISSN: 2329-8731

Journal of Infectious Diseases & Preventive Medicine
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

No Evidence of Interspecific Genetic Exchange by Multi-Locus Microsatellite Typing Between Leishmania Killicki and Leishmania Major in a Mixed Focus of Cutaneous Leishmaniasis in Southeast Tunisia

Myriam Harrabi1,2*, Wissem Ghawar1, Mallorie Hide3, Jihène Bettaieb1, Rihab Yazidi1, Kaouthar Jaouadi1, Chaâbane Sana1, Bilel Chalgha1, Amine Toumi1,Amor Zaâtour1, Mohamed Raouène4, Anne-Laure Bañuls3 and Afif Ben Salah1

1Institut Pasteur, 13, Place Pasteur, Service du Laboratoire d’Épidémiologie Médicale et Laboratoire de Transmission, Tunisia

2Faculté des Sciences de Bizerte-7021 Jarzouna-Université de Carthage, Tunis, Tunisia

3UMR MIVEGEC, Centre IRD de Montpellier 911, Montpellier, France

4Direction Régionale de la santé de Tataouine- 3263 Tataouine Nouvelle- Tunisie, Tunisia

*Corresponding Author:
Myriam Harrabi
Institut Pasteur, 13, Place Pasteur
Service du Laboratoire d’Épidémiologie Médicale et Laboratoire de Transmission
Contrôle et Immunobiologie des Infections, Tunisia
Tel: +216 23 400 448
E-mail: [email protected]

Received Date: March 16, 2017; Accepted Date: May 10, 2017; Published Date: May 17, 2017

Citation: Harrabi M, Ghawar W, Hide M, Bettaieb J, Yazidi Y, et al. (2017) No Evidence of Interspecific Genetic Exchange by Multi-Locus Microsatellite Typing Between Leishmania Killicki and Leishmania Major in a Mixed Focus of Cutaneous Leishmaniasis in Southeast Tunisia. J Infect Dis Preve Med 5:163. doi: 10.4172/2329-8731.1000163

Copyright: © 2017 Harrabi M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Sixty-four Leishmania samples were isolated from patients in several villages in the Tataouine governorate, southeast Tunisia. This region is known to be a mixed focus of human cutaneous Leishmaniasis caused by Leishmania (L.) killicki (synonymous L. tropica) and L. major. To identify the Leishmania species in this governorate, a nested polymerase chain reaction based on the variable region of the kinetoplast minicircle was performed on each isolate. Multi-locus microsatellite typing using markers selected for their ability to amplify the two species was used to explore patterns of interspecific genetic exchange. Thirteen L. major and 51 L. killicki isolates were identified. The analysis of microsatellite data showed very low genetic diversity in each species with this set of microsatellites but a high differentiation between the two species. Nine L. major and five L. killicki strains revealed heterozygous genotypes with no shared allele between the two species. These heterozygotes probably resulted from genetic mutation events and not from interspecific genetic exchange. Specific and different epidemiological cycles at the sympatric level might explain the absence of genetic exchange between the two Leishmania species in the Tataouine governorate.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]online.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version