No Evidence of Interspecific Genetic Exchange by Multi-Locus Microsatellite Typing Between Leishmania Killicki and Leishmania Major in a Mixed Focus of Cutaneous Leishmaniasis in Southeast Tunisia
Myriam Harrabi1,2*, Wissem Ghawar1, Mallorie Hide3, Jihène Bettaieb1, Rihab Yazidi1, Kaouthar Jaouadi1, Chaâbane Sana1, Bilel Chalgha1, Amine Toumi1,Amor Zaâtour1, Mohamed Raouène4, Anne-Laure Bañuls3 and Afif Ben Salah1
- *Corresponding Author:
- Myriam Harrabi
Institut Pasteur, 13, Place Pasteur
Service du Laboratoire d’Épidémiologie Médicale et Laboratoire de Transmission
Contrôle et Immunobiologie des Infections, Tunisia
Tel: +216 23 400 448
E-mail: [email protected]
Received Date: March 16, 2017; Accepted Date: May 10, 2017; Published Date: May 17, 2017
Citation: Harrabi M, Ghawar W, Hide M, Bettaieb J, Yazidi Y, et al. (2017) No Evidence of Interspecific Genetic Exchange by Multi-Locus Microsatellite Typing Between Leishmania Killicki and Leishmania Major in a Mixed Focus of Cutaneous Leishmaniasis in Southeast Tunisia. J Infect Dis Preve Med 5:163. doi: 10.4172/2329-8731.1000163
Copyright: © 2017 Harrabi M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sixty-four Leishmania samples were isolated from patients in several villages in the Tataouine governorate, southeast Tunisia. This region is known to be a mixed focus of human cutaneous Leishmaniasis caused by Leishmania (L.) killicki (synonymous L. tropica) and L. major. To identify the Leishmania species in this governorate, a nested polymerase chain reaction based on the variable region of the kinetoplast minicircle was performed on each isolate. Multi-locus microsatellite typing using markers selected for their ability to amplify the two species was used to explore patterns of interspecific genetic exchange. Thirteen L. major and 51 L. killicki isolates were identified. The analysis of microsatellite data showed very low genetic diversity in each species with this set of microsatellites but a high differentiation between the two species. Nine L. major and five L. killicki strains revealed heterozygous genotypes with no shared allele between the two species. These heterozygotes probably resulted from genetic mutation events and not from interspecific genetic exchange. Specific and different epidemiological cycles at the sympatric level might explain the absence of genetic exchange between the two Leishmania species in the Tataouine governorate.