Nodaviruses in Wild Fish Population Collected Around Aquaculture Cage Sites from Coastal Areas of TunisiaNadia Cherif* and Amdouni Fatma
Aquaculture laboratory, National Institute of Sea Science and Technologies, 28 Rue de 2 Mars, 2025, Tunisia
- *Corresponding Author:
- Nadia C
Aquaculture laboratory, National Institute of Sea Science and Technologies
28 Rue de 2 Mars, 2025, Tunisia
Tel: +00216 71 735 848
E-mail: [email protected]; [email protected]
Received date: June 15, 2017; Accepted date: June 30, 2017; Published date: July 07, 2017
Citation: Nadia C, Amdouni F (2017) Nodaviruses in Wild Fish Population Collected Around Aquaculture Cage Sites from Coastal Areas of Tunisia. Fish Aqua J 8: 209. doi:10.4172/2150-3508.1000209
Copyright: © 2017 Nadia C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This report describes the viral epidemiology of wild fish adjacent to cage farms within the Tunisian coasts and is focused on viral nervous necrosis virus (VNNV). A total of 92 apparently healthy wild marine fish were collected near aquaculture facilities in five different coastal areas of Tunisia. The brains and eyes of fish were examined by quantitative real time reverse transcriptase-polymerase chain reaction (qRT-PCR) to detect the nodavirus coat protein gene of. A total of 57 out of 92 (61.9%) samples were positive for nodavirus by qRT-PCR. This finding indicates that carrier fish occur at a considerable level in populations of wild marine fish. Samples from 13 fish species were found to be positive to the virus genome: Sarpa salpa, Trachurs trachurus, Boobs boops, Sardinella aurita, Diplodus vulgaris, Diplodus puntazzo Liza aurata, Diplodue sargus, Sparus aurata, Sardina pilchardus, Spicara maena, Spondyliosoma cantharus, and Diplodus annularis. The partial sequences of the RNA2 coat protein gene of these strains were identical with RGNNV type previously identified within farmed sea bass and sea bream species in Tunisia, with a homology >97%. With respect to the proximity of the sampling sites to the coast and to rearing facilities, results analysis can suggest that these viruses may be indigenous to Tunisian coastal waters.