alexa Non-Edible Vernonia galamensis Oil and Mixed Bacterial Cultures for the Production of Polyhydroxyalkanoates
ISSN: 2329-6798

Modern Chemistry & Applications
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Research Article

Non-Edible Vernonia galamensis Oil and Mixed Bacterial Cultures for the Production of Polyhydroxyalkanoates

Adrian D Allen1,4*, Folahan O Ayorinde2 and Broderick E Eribo3
1Department of Comprehensive Sciences, Howard University, Washington, DC, USA
2Department of Chemistry, Howard University, Washington, DC, USA
3Department of Biology, Howard University, Washington, DC, USA
4NOAA Center for Atmospheric Sciences, Howard University, Washington, DC, USA
Corresponding Author : Adrian Douglas Allen
Department of Comprehensive Sciences and NOAA Center for Atmospheric Sciences
Howard University, 415 College St. NW, Washington, DC, 20059, USA|
Tel: 202-806-4172
E-mail: [email protected]
Received July 22, 2014; Accepted September 18, 2014; Published September 22, 2014
Citation:Allen AD, Ayorinde FO, Eribo BE (2014) Non-Edible Vernonia galamensis Oil and Mixed Bacterial Cultures for the Production of Polyhydroxyalkanoates. Mod Chem appl 2:136. doi: 10.4172/2329-6798.1000136
Copyright:2014 Allen AD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Since the oil crisis of the 1970s much attempt has been made, albeit with varying degrees of success, to source the ideal substrate and bacteria for the production of PHA. The non-edible, naturally epoxidized seed oil from Vernonia galamensis and mixed cultures consisting of Alcaligenes latus (ATCC 29712), Cupriavidus necator (ATCC 17699), Escherichia coli (DH5α) and Pseudomonas oleovorans (ATCC 29347), were evaluated for PHA production under batch and fed-batch fermentations. PHA production, optimized by the mixed culture of E. coli and C. necator, was 0.4-19% (%wt/wt, cdw) for batch and fed-batch fermentations. Analyses of PHA by Matrix Assisted Laser Desorption Ionization- Time of Flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography Mass Spectrometry (GC/MS) identified the 3-hydroxybutyrate (3HB) monomeric unit. The PHA ester bond stretching vibration (C=O), was confirmed at absorption 1740.66 cm-1, using Fourier Transform Infrared Spectroscopy (FTIR). Gel Permeation Chromatography (GPC) indicated peak molecular weights between 3.8×103-1.12×106 Da with melting points (Tm), 60-90°C. The data further illustrates that inedible oils could be the ideal carbon source for the production of PHA.

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