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Non-thermal Effects of Far-Infrared Ray(FIR) on Human Hepatocellular Carcinoma Cells HepG2 and their Tumors | OMICS International | Abstract
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

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Research Article

Non-thermal Effects of Far-Infrared Ray(FIR) on Human Hepatocellular Carcinoma Cells HepG2 and their Tumors

Tatsuo Ishikawa1, Jun Ishibashi2, Kikuji Yamashita1*, Shine-Od Dalkhsuren1, Kaori Sumida1, Takahumi Masui1 and Seiichiro Kitamura1

1Department of Oral and Maxillofacial Anatomy, Medical Science for Oral and Maxillofacial Regeneration, Graduate School of Health Biosciences, University of Tokushima, 3-18-15 Kuramoto, Tokushima, 770-8504 Japan

2Blanka Dental Office

*Corresponding Author:
Dr. Kikuji Yamashita,
Department of Oral and Maxillofacial Anatomy
Medical Science for Oral and Maxillofacial Regeneration
Graduate School of Health Biosciences
University of Tokushima, 3-18-15 Kuramoto
Tokushima, 770-8504 Japan
Fax :
E-mail : [email protected]

Received Date: November 03, 2009; Accepted Date: December 29, 2009; Published Date: December 29, 2009

Citation: Ishikawa T, Ishibashi J, Yamashita K, Dalkhsuren SO, Sumida K, et al. (2009) Non-thermal Effects of Far-Infrared Ray (FIR) on Human Hepatocellular Carcinoma Cells HepG2 and their Tumors. J Cancer Sci Ther 1: 078-082. doi:10.4172/1948-5956.1000012

Copyright: © 2009 Ishikawa T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Background: We developed a cell culture CO2 incubatorand a mice rack that can continuously irradiate cells ormurine with FIR. Our goal is to make clear the non-thermaleffect of FIR on HepG2 with these instrumentsmorphologically.

Methods: By using them, in vitro , we examined theproliferation of cultured HepG2 cells with hematocytometer,BrdU assay, WST-1 assay, HE staining, Toluidine bluestaining and microarray studies. And in vivo, we measuredthe tumors, observed the sections by IHC, DAPI stainingwith light microscopes and performed microarray studies.

Results: Proliferation of HepG2 cells were suppressed(e.g., cell count declined by 34% after 10 days of FIRirradiation), tumor volumes reduced by 86% after 30 daysof FIR irradiation, mRNA of Vascular Endothelial GrowthFactor (VEGF) decreased by 48%, vascular area in crosssections from the tumors decreased 60% compared withthe control. More frequent properties in apoptosis wereobserved by TUNEL and DAPI staining in FIR-treatedgroups. Body weight of mice increased compared with thecontrol. Oxydation and Reduction (Redox) reactions byH+ (proton and electron)/O2- (a kind of Reactive OxygenSpecies (ROS)) were induced by FIR.

Conclusions: These results clarified that FIR inhibitedthe proliferation of HepG2 at non-thermal circumstances(at 25±0.5, 37±0.5°C). FIR will serve as a tool againstdiseases induced by HepG2.


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