alexa Novel Antiproliferative and Antioxidant Role of BjAnn1, a Mustard Annexin Protein in Human Glioblastoma Cell Lines
ISSN: 1948-5956

Journal of Cancer Science & Therapy
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Research Article

Novel Antiproliferative and Antioxidant Role of BjAnn1, a Mustard Annexin Protein in Human Glioblastoma Cell Lines

Gangadhara R Sareddy1#, Divya Kesanakurti2#, Pulugurtha Bharadwaja Kirti3* and Phanithi Prakash Babu1*
1Department of Biotechnology, School of Life Sciences, University of Hyderabad, Hyderabad, India
2Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine, Peoria, IL, USA
3Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India
#Both the authors contributed equally
Corresponding Author : Phanithi Prakash Babu
Professor and Head, Department of Biotechnology
School of Life Sciences, University of Hyderabad
Hyderabad, India-500046
Tel: 0091-40-23134584; 23013336
Fax: 0091-40-23010120
E-mail: [email protected], [email protected]
  Pulugurtha Bharadwaja Kirti
Department of Biotechnology, School of Life Sciences
University of Hyderabad, Hyderabad, India-500046
Tel: 0091-40-23134584; 23013336
Fax: 0091-40-23010120
Received April 27, 2013; Accepted June 20, 2013; Published June 24, 2013
Citation: Gangadhara Reddy S, Divya K, Kirti PB, Prakash Babu P (2013) Novel Antiproliferative and Antioxidant Role of BjAnn1, a Mustard Annexin Protein in Human Glioblastoma Cell Lines. J Cancer Sci Ther 5: 256-263. doi:10.4172/1948-5956.1000214
Copyright: © 2013 Gangadhara Reddy S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Glioblastoma represents the deadliest tumors of the central nervous systems and their bleak prognosis remains unchanged over the past few decades and requiring new treatments that curb the tumor progression. Cancer cells possess high reactive oxygen species (ROS) levels when compared to their normal control counterparts. We have earlier characterized the ROS-scavenging annexin protein, BjAnn1 from Brassica juncea and demonstrated its peroxidase activity. Since annexins are widely conserved across evolutionary lines with involvement in stress alleviation, we attempted to verify the effect of ectopic expression of ROS-scavenging BjAnn1 in human glioblastoma cell lines. The multiple sequence alignment revealed that BjAnn1 showed homology to human annexin A13 (73.18%), annexin A2 (70.66%), annexin A3 (72.53%) and annexin A8 (70.03%). Stable expression of BjAnn1 in U251 and U87 cells inhibited proliferation, migration and invading abilities in correlation with significant decrease in ROS levels in comparison to the empty vector (EV)-expressing controls. Multiple gene-profiling using cDNAPCR arrays revealed a prominent transcriptional upregulation of oxidative stress responsive genes including CCS, CYB, DUOX2, FOXM, GSS, MBL2, MT3, MTL5, NME5, PXDN, SOD2 and SOD3 in BjAnn1-expressing glioblastoma cells. Western blotting confirmed the significant increase in the expression of antioxidant enzymes SOD2 (MnSOD) and SOD3 (Cu/ZnSOD) in BjAnn1-expressing cells. We also observed a significant inhibition in nuclear translocation of p65 and p50, NF-κB reporter activity and expression of NF-κB-target genes in BjAnn1 expressing cells. In summary, our study shows a prominent tumor-suppressive role of ROS-scavenging BjAnn1 in glioblastoma cell lines suggesting it as a novel candidate for efficient gene therapy in glioblastoma.

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