alexa Novel Isozyme-Specific Quantitation Method for Alpha-Amylases in Human Plasma Revealed Possible AMY2B Induction by Alpha-Amylase Inhibitor, CS-1036
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Novel Isozyme-Specific Quantitation Method for Alpha-Amylases in Human Plasma Revealed Possible AMY2B Induction by Alpha-Amylase Inhibitor, CS-1036

Mayumi Hayashi1, Toshiyuki Kosaka2, Mitsunori Kato1, Tomohiro Honda3, Takuo Washio4, Atsushi Yamasaki5, Kazuishi Kubota1* and Akira Shinagawa1

1Discovery Science and Technology Department, Daiichi Sankyo RD Novare Co., Ltd, Japan

2Translational Medicine and Clinical Pharmacology Department, Daiichi Sankyo Co., Ltd, Japan

3Drug Discovery Technology Function, Asubio Pharma Co., Ltd, Japan

4Asia Development Department, Daiichi Sankyo Co., Ltd, Japan

5Daiichi Sankyo Pharma Development, Daiichi Sankyo, Inc, Japan

*Corresponding Author:
Kazuishi Kubota
Discovery Science and Technology Department
Daiichi Sankyo RD Novare Co., Ltd, Japan
Tel: +81-80-4468-2489
E-mail: [email protected]

Received Date: February 22, 2017; Accepted Date: March 28, 2017; Published Date: April 04, 2017

Citation: Hayashi M, Kosaka T, Kato M, Honda T, Washioi T, et al. (2017) Novel Isozyme-Specific Quantitation Method for Alpha-Amylases in Human Plasma Revealed Possible AMY2B Induction by Alpha-Amylase Inhibitor, CS-1036. J Proteomics Bioinform 10:108-113. doi: 10.4172/jpb.1000430

Copyright: © 2017 Hayashi M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In a clinical trial of an α-amylase inhibitor (CS-1036), which was developed for the treatment of patients with type 2 diabetes, the half-life of plasma CS-1036 concentration was prolonged with the increase of the dose level. The prolonged half-life was assumed to be associated with alternation in the plasma level of any one of the α-amylase isozymes from the treatment. Human α-amylase is classified into 3 isozymes encoded by AMY1A, AMY2A, and AMY2B. Due to high sequence homology between α-amylase isozymes and the low plasma level, it is extremely challenging to quantify individual isozymes. A mass spectrometry-based approach, Multiple Reaction Monitoring (MRM) using the Absolute Quantification (AQUA) strategy, has been applied to quantification of target proteins, and this approach provides high selectivity and specificity. Here we report a novel quantitation method of α-amylase isozymes, which is a combination of purification of α-amylase from plasma by starch affinity adsorption and LCMRM- MS. This method was applied to the plasma samples from the clinical trial of CS-1036. As a result, only AMY2B showed a statistically significant increase in a time-dependent manner. This result suggested that AMY2B might be related to the prolonged half-life of CS-1036.

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