alexa Occurrence of Agrobacterium Vitis Carrying Two Opine-Type Plasmids in Tunisian Vineyards
ISSN: 2157-7471

Journal of Plant Pathology & Microbiology
Open Access

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Research Article

Occurrence of Agrobacterium Vitis Carrying Two Opine-Type Plasmids in Tunisian Vineyards

Samir Chebil1, Rabeb Fersi1*, Synda Chenenaoui1, Emna Abdellatif1, Giuseppe Durante2, Elena Zacchi2, Ali Rhouma3 and Ahmed Mliki1

1Laboratory of Plant Molecular Physiology, Biotechnology Center of Borj-cédria (CBBC), Hammam-lif, Tunis, Tunisia

2International Plant Analysis and Diagnostics s.r.l, Via Einstein, località Cascina Codazza 26900 LODI (LO), Italy

3Laboratory of Protection and Improvement of genetic Resources of Olive, Olive Tree Institute, Avenue H. Karray, 1002 Tunis Belvedere, Tunis, Tunisia

*Corresponding Author:
Rabeb Fersi
Laboratory of Plant Molecular Physiology
Biotechnology Center of Borj-cédria (CBBC)
Hammam-lif, Tunis, Tunisia
Tel:(+216)27890245
E-mail: [email protected]

Received date: April 01, 2013; Accepted date: May 07, 2013; Published date: May 15, 2013

Citation: Chebil S, Fersi R, Chenenaoui S, Abdellatif E, Durante G, et al. (2013) Occurrence of Agrobacterium Vitis Carrying Two Opine-Type Plasmids in Tunisian Vineyards. J Plant Pathol Microb 4:175. doi:10.4172/2157-7471.1000175

Copyright: © 2013 Chebil S. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Fifty DNA samples were recovered from infected wood tissue of 1 and 2-year-old grapevine (Vitis vinifera L.) cultivars collected from several vineyards located in the Center of Tunisia. The strains were differentiated using a Multiplex PCR assay with combination of pehA, virF and virD2 gene-specific primers which amplified the corresponding fragments from nearly all strains and distinguished clearly between Agrobacterium vitis and A. tumefaciens strains carrying octopine or nopaline pTis and A. vitis vitopine strains. The isolates were segregated into three main groups, the first group carries octopine type Ti plasmids; the second vitopine type Ti plasmids and the third group both octopine and vitopine type Ti plasmids. The polygalacturonase gene sequence from 10 isolates showed 94-97% identity to the sequences of A. vitis previously deposited in NCBI GenBank database. Obtained nucleotide sequences were submitted to Genbank under accession numbers from JX946285 to JX946294.

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