alexa On-line SPE of Three Steroids with a Novel Polymeric Monolith
ISSN: 2471-2698

Pharmaceutical Analytical Chemistry: Open Access
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Research Article

On-line SPE of Three Steroids with a Novel Polymeric Monolith

Haiyan Liu1,2*, Hang Zhang1,2, Shuai He1, Haonan Li1 and Ligai Bai1,2

1College of Pharmaceutical Sciences of Hebei University, Baoding, China

2Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding, China

*Corresponding Author:
Haiyan Liu
College of Pharmaceutical Sciences of Hebei University
Baoding, Hebei 071002, China
Tel: 0086 312 5971107
Fax: 0086 312 5971107
E-mail: [email protected]

Received date: October 15, 2015 Accepted date: October 29, 2015 Published date: October 31, 2015

Citation: Liu H, Zhang H, He S, Li H, Bai L (2015) On-line SPE of Three Steroids with a Novel Polymeric Monolith. Pharm Anal Chem Open Access 1:104. doi: 10.4172/2471-2698.1000104

Copyright: 2015 Liu H. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In this report, a novel poly (N-isopropyl acrylamide-glycidyl methacrylate-ethylene glycol dimethacrylate) monolithic column was introduced for solid phase extraction. The monolith was prepared using N-isopropyl acrylamide and glycidyl methacrylate as the functional monomers, ethylene glycol dimethacrylate as the cross linker. In order to evaluate the mechanical stability and permeability of the material, the pressure drop across the column was measured in different flow-rates. Moreover, the monolith was used as on-line solid phase extraction material and coupled to high performance liquid chromatography to determine dexamethasone acetate, norgestrel and halcinonide in urine samples.

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