alexa Optimisation of Medium Formulation and Growth Conditions for Xylanase Production by Aspergillus brasiliensis in Submerged Fermentation (SmF) | OMICS International | Abstract
ISSN: 2376-0214

Journal of Biodiversity, Bioprospecting and Development
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Optimisation of Medium Formulation and Growth Conditions for Xylanase Production by Aspergillus brasiliensis in Submerged Fermentation (SmF)

Hooi Ling Ho* and Jamila Said Hood
Faculty of Applied Sciences, UCSI University, 1, Jalan Menara Gading, UCSI Heights, Cheras, 56000 Kuala Lumpur, Malaysia.
*Corresponding Author : Hooi Ling Ho
Faculty of Applied Sciences
UCSI University, 1, Jalan Menara Gading
UCSI Heights, Cheras, 56000 Kuala Lumpur, Malaysia
Tel: +6012 369 9854
Fax: +603 9102 2614
E-mail: [email protected]
Received February 25, 2014; Accepted April 14, 2014; Published April 25, 2014
Citation: Ho HL, Hood JS (2014) Optimisation of Medium Formulation and Growth Conditions for Xylanase Production by Aspergillus brasiliensis in Submerged Fermentation (SmF). J Biodivers Biopros Dev 1:102. doi:10.4172/2376-0214.1000102
Copyright: © 2014 Ho HL et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Objective: There has been much concern and immense importance on xylanase due to its potential industrial applications in the manufacturing of pulp and paper, baking, food and beverages, textile and animal feeds production. There are continuously endeavors to turn xylanase into a profitable market with lower production costs using optimum fermentation medium and growth conditions. Thus, the main objectives of this study were to determine the optimum medium formulation and growth conditions for the production of xylanase by Aspergillus brasiliensis ATCC 16404 and to elucidate the correlation of xylanase synthesis with biomass, pH and protein concentration in submerged fermentation (SmF).

Methods: The effect of various carbon sources: sucrose, glucose, maltose, galactose, glycerol, lactose and ethanol and nitrogen sources: yeast extract, urea, peptone, ammonium sulphate, ammonium nitrate, ammonium chloride and sodium nitrate on xylanase activity were examined to obtain the optimum medium formulation for xylanase activity by A. brasiliensis. In addition, growth temperatures of 28 to 36°C and initial pH medium of 3 to 11 were also examined to elucidate the optimum growth conditions for the production of xylanase.

Results: The highest xylanase activity of 39.567 U/mL at 72 h was obtained using sucrose and yeast extract as the optimised carbon and nitrogen sources. Our results also showed that 30°C and pH 6.5 were the optimum growth condition of A. brasiliensis for xylanase synthesis. 38.528 U/mL of xylanase activity was observed at 72 h when the initial pH medium was adjusted to 5.0. Nevertheless, when the initial pH medium was increased to 6.5, the xylanase synthesis increased almost 3% at 72 h.

Conclusion: Hence, in our study, the optimum medium formulation and growth conditions for the production of xylanase by A. brasiliensis in SmF was achieved by growing the fungi in medium containing sucrose and yeast extract with the initial medium pH 6.5 at 30°C.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7