alexa Optimisation of Rhamnolipid: A New Age Biosurfactant fr
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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Research Article

Optimisation of Rhamnolipid: A New Age Biosurfactant from Pseudomonas aeruginosa MTCC 1688 and its Application in Oil Recovery, Heavy and Toxic Metals Recovery

Pathaka AN1 and Pranav H Nakhate2*
1Research Dean and Head of Institution, Amity Institute of Biotechnology, Amity University Rajasthan, India
2Biochemical Engineering Scholar Jaipur, Amity Institute of Biotechnology, Amity University Rajasthan, India
Corresponding Author : Pranav H Nakhate
Biochemical Engineering Scholar
mity University Rajasthan, 14-Gopal Bari
Ajmer Road, Jaipur 302001, India
Tel: 950-357-3593
E-mail: [email protected]
Received April 20, 2015; Accepted May 22, 2015; Published May 27, 2015
Citation: Pathaka AN, Nakhate PH (2015) Optimisation of Rhamnolipid: A New Age Biosurfactant from Pseudomonas aeruginosa MTCC 1688 and its Application in Oil Recovery, Heavy and Toxic Metals Recovery. J Bioprocess Biotech 5:229. doi: 10.4172/2155-9821.1000229
Copyright: © 2015 Pathaka AN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Rhamnolipid is a new age biosurfactant, commonly produced biotechnologically with Pseudomonas aeruginosa in batch cultivations whereas novel substrates like karanja oil and soybean oil cake were employed, giving yield of 3.609 gm/lit of rhamnolipid, which shows effective and enhanced production of rhamnolipid, compared to other vegetable oil as a carbon sources, mentioned in the literature, at optimised pH of 7.0 and optimised substrate concentration at 3.0%. The optimum yield in terms of substrate was observed as 3.609 gm/lit of rhamnolipid produced per 5.255 ml of oil consumed, while yield in terms of biomass was observed as 3.609 gm/lit of rhamnolipid produced per 2.5 gm of dry biomass. The chloroform:methanol (2:1) extraction system was found to be the best solvent extraction system, where 83% of the rhamnolipid was recovered. The Rhamnolipid was successfully applied for the heavy and toxic metals recovery, where rhamnolipid reduces heavy metal concentration to 73%, 65% and 71% for FeCl3, ZnSO4 and Pb(NO3)2 respectively, while 43% in the case of toxic metal i.e. NaF. The produced rhamnolipid was found efficient in recovering 31% no-edible oil from oil sludge.

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