Optimization of Bio-Mosquitocidal Production using Local Bacterial Strains of Bacillus Sphaericus
Ferial M Rashad*, Waleed D Saleh and Hayam M Fathy
Department of Microbiology, Faculty of Agriculture, Cairo Univ. Giza, Egypt
- *Corresponding Author:
- Ferial M Rashad
Department of Microbiology, Faculty of Agriculture
Cairo University Giza, Egypt
Received date: May 16, 2013; Accepted date: June 27, 2013; Published date: July 01, 2013
Citation: Rashad FM, Saleh WD, Fathy HM (2013) Optimization of Bio- Mosquitocidal Production using Local Bacterial Strains of Bacillus Sphaericus. J Biofertil Biopestici 4:134. doi:10.4172/2155-6202.1000134
Copyright: © 2013 Rashad FM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Highly active local strains of B. sphaericus were used along with the reference strains 1593 and 2297 to optimize the biocide production. The optimum conditions were as follow: 20% of total flask volume, pH 7.0,inoculum size 3-5×104 cfu / ml, 30° C and 200 rpm/5 days. Glycerol was the best C source at 0.5%; a greatsuppression up to >80% with propionic acid. All tested strains failed to synthesize biocide in the presence of inorganic N as a sole source; beef extract gave the supreme yield. Mg2+ was necessary for maximal productivity;however, Ca2+ damaged the fermentation. The uppermost productivity was achieved from 25 g/l fodder yeast, 500 ml/l corn steep liquor and 0.3 g/l MgSO4 as a medium. Chicken feather at 30 g/l proved to be an excellent monocomponent; a superlative improvement was achieved when feather (3.0%) supplemented with 0.03% of MgSO4.