Optimization of Submerged Culture Conditions for Exo-Polysaccharides Production by Streptomyces Nasri-UV 135 in Bioreactor
1Mubarak City for Scientific Research and Technology Applications, Genetic Engineering and Biotechnology Research Institute (GEBRI), Bioprocess Development Dept, New Bourg El-Arab City, 21934 Alexandria, Egypt
2Mubarak City for Scientific Research and Technology Applications, Genetic Engineering and Biotechnology Research Institute (GEBRI), Pharmaceuticals Bioproducts Dept, New Bourg El-Arab City, 21934 Alexandria, Egypt
- *Corresponding Author:
- Dr. Usama Beshay
Dept. Genetic Engineering & Biotechnology Research Institute (GEBRI)
Mubarak City for Scientific Research and Technology Applications (MUCSAT)
New Bourg El-Arab City, Universities and Research District
21934 Alexandria, Egypt
Fax : +2034593423
E-mail : [email protected], [email protected]
Received Date: November 08, 2012; Accepted Date: December 17, 2012; Published Date: December 19, 2012
Citation: Beshay U, Daba A, Gohar Y (2009) Optimization of submerged culture conditions for exo-polysaccharides production by Streptomyces nasri-UV 135 in bioreactor. J Microbial Biochem Technol 1: 043-046. doi:10.4172/1948-5948.1000008
Copyright: © 2013 Jung YG. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Production of Exopolysaccharides ?EPS? and microbial biomass by Streptomyces nasri were influenced by the type of carbon source (glycerol, xylose, fructose, galactose, glucose, mannose, sucrose, lactose, maltose, dextrin, soluble starch, corn starch and potato starch) and the nitrogen source (glycine, aspartic acid, glutamic acid, proline, ammonium sulphate, sodium nitrate and beef extract ) used in the medium. Xylose and glycine were the most suitable sources of carbon and nitrogen, respectively, for both production of EPS and mycelial growth. The highest EPS production was obtained in a medium containing (g/l) 30 xylose, 2.7 glycine, 4.0 NaCl, 0.5 MgSO4, 1.0 K2HPO4, and 1.0 CaCO3. Exopolysaccharides production and mycelial growth in the above suggested medium were significantly increased in a 3-l stirred tank bioreactor, where the maximum EPS concentration was 8.73 g/l, which was an approximately 1.6 time higher than that in shake culture.