alexa Optimization of Submerged Culture Conditions for Exo-Po
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Optimization of Submerged Culture Conditions for Exo-Polysaccharides Production by Streptomyces Nasri-UV 135 in Bioreactor

Usama Beshay1*, Ayman Daba2, Yousry Gohar3

1Mubarak City for Scientific Research and Technology Applications, Genetic Engineering and Biotechnology Research Institute (GEBRI), Bioprocess Development Dept, New Bourg El-Arab City, 21934 Alexandria, Egypt

2Mubarak City for Scientific Research and Technology Applications, Genetic Engineering and Biotechnology Research Institute (GEBRI), Pharmaceuticals Bioproducts Dept, New Bourg El-Arab City, 21934 Alexandria, Egypt

3Alexandria University, Faculty of Science, Division of Microbiology, Alexandria, Egypt

*Corresponding Author:
Dr. Usama Beshay
Bioprocess Development
Dept. Genetic Engineering & Biotechnology Research Institute (GEBRI)
Mubarak City for Scientific Research and Technology Applications (MUCSAT)
New Bourg El-Arab City, Universities and Research District
21934 Alexandria, Egypt
Fax : +2034593423
E-mail : [email protected], [email protected]

Received Date: November 08, 2012; Accepted Date: December 17, 2012; Published Date: December 19, 2012

Citation: Beshay U, Daba A, Gohar Y (2009) Optimization of submerged culture conditions for exo-polysaccharides production by Streptomyces nasri-UV 135 in bioreactor. J Microbial Biochem Technol 1: 043-046. doi:10.4172/1948-5948.1000008

Copyright: © 2013 Jung YG. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Production of Exopolysaccharides ?EPS? and microbial biomass by Streptomyces nasri were influenced by the type of carbon source (glycerol, xylose, fructose, galactose, glucose, mannose, sucrose, lactose, maltose, dextrin, soluble starch, corn starch and potato starch) and the nitrogen source (glycine, aspartic acid, glutamic acid, proline, ammonium sulphate, sodium nitrate and beef extract ) used in the medium. Xylose and glycine were the most suitable sources of carbon and nitrogen, respectively, for both production of EPS and mycelial growth. The highest EPS production was obtained in a medium containing (g/l) 30 xylose, 2.7 glycine, 4.0 NaCl, 0.5 MgSO4, 1.0 K2HPO4, and 1.0 CaCO3. Exopolysaccharides production and mycelial growth in the above suggested medium were significantly increased in a 3-l stirred tank bioreactor, where the maximum EPS concentration was 8.73 g/l, which was an approximately 1.6 time higher than that in shake culture.

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