alexa Optimization of Submerged Culture Conditions for Mycelial Growth and Extracellular Polysaccharide Production by Coriolus versiolor
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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Research Article

Optimization of Submerged Culture Conditions for Mycelial Growth and Extracellular Polysaccharide Production by Coriolus versiolor

Feifei Wang1,3, Jianchun Zhang1,2*, Limin Hao1,2, Shiru Jia3, Jianming Ba4 and Shuang Niu1,3
1The Research Center of China Hemp Materials, Beijing 100027, P. R. China
2The Quartermaster Equipment Institute of GLD of PLA, Beijing 100010, P. R. China
3Key Laboratory Industrial Fermentation Microbiology, University of Science & Technology, Tianjin 300457, P. R. China
4Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, P. R. China
Corresponding Author : Jianchun Zhang
The Research Center of China Hemp Materials
Beijing 100027,China
Tel: + 86-10-65250502
E-mail: [email protected]
Received April 09, 2012; Accepted June 04, 2012; Published June 06, 2012
Citation: Wang F, Zhang J, Hao L, Jia S, Ba J, et al. (2012) Optimization of Submerged Culture Conditions for Mycelial Growth and Extracellular Polysaccharide Production by Coriolus versiolor. J Bioprocess Biotech 2:124. doi: 10.4172/2155-9821.1000124
Copyright: © 2012 Wang F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

 This paper is concerned with optimization of submerged culture conditions for mycelial growth and Exopolysaccharides (EPS) yield with Coriolus versiolor by both one-factor-at-a-time and orthogonal matrix methods. Glucose and yeast-Extracts were identified to be the most suitable carbon and nitrogen sources, respectively. The optimal initial pH, inoculum size and liquid volume for mycelial growth and EPS yield were 5.0, 8% and 150mL/500mL, respectively. Subsequently, the concentration of glucose, yeast-extract, KH2PO4, and MgSO4•7H2O were optimized using the orthogonal matrix method. The effects of media composition on the mycelial growth of Coriolus versiolor were in the order of glucose >KH2PO4 > yeast-extract > MgSO4•7H2O, and those on EPS yield were in the order of glucose >MgSO4•7H2O> yeast-extract >KH2PO4.The optimal concentrations for enhanced yield were determined as 30 g/L glucose, 7.0 g/L yeast-extract, 1.0 g/L KH2PO4, 1.0 g/L MgSO4•7H2O, and 40 g/L glucose, 6.0 g/L yeast-extract, 1.0 g/L KH2PO4, 1.5 g/L MgSO4•7H2O for mycelial and EPS yield, respectively. The verification experiments confirmed the final medium. This optimization strategy (30 g/L glucose, 7.0 g/L yeast-extract, 2.0 g/L KH2PO4 and 0.5 g/L MgSO4•7H2O) in shake flask culture lead to a mycelial yield of 5.18 g/L, and EPS yield of 0.64 g/L, respectively, which were considerably higher than those obtained in preliminary studies. Under optimal culture conditions, the maximum EPS concentration in a 5-L stirred-tank bioreactor was 0.75 g/L, while the maximum mycelial yield was 8.55 g/L. This also corresponded to 14.67% and 39.42% enhancement in EPS yield and mycelial dry weight, respectively, compared with the verification test results.

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