OPTIMIZATION OF SUPERCRITICAL CARBON DIOXIDE EXTRACTION OF ESSENTIAL OIL FROM CINNAMOMUM TAMALA ALONG WITH ITS CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY
Essential oil was extracted from leaves of Cinnamomum tamala by four different methods; Traditional hydro distillation (HD), solvent extraction (SE), ultrasonication (US) and supercritical carbon dioxide extraction (SC-CO2). The optimization process was carried out using factorial design and maximum yield (3.64%) of essential oil was obtained at optimum conditions of pressure (200 bar), temperature (55 0C) and CO2 flow rate (15 g/min). Essential oil extracted by SC-CO2 was found to be best in quantity and quality. Chemical composition of essential oils obtained from leaves Cinnamomum tamala were analysed by GC/MS and its antioxidant activity was also studied.