alexa Optimization of Total Carotenoid Production by Halorubrum Sp. TBZ126 Using Response Surface Methodology | OMICS International | Abstract
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Optimization of Total Carotenoid Production by Halorubrum Sp. TBZ126 Using Response Surface Methodology

Masoud Hamidi1,2, Malik Zainul Abdin3, Hossein Nazemyieh1, Mohammad Amin Hejazi4* and Mohammad Saeid Hejazi1,5*

1Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

2Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

3Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Jamia Hamdard (Hamdard University), New Delhi, India

4West and Northwest Agricultural Biotechnology Research Institute of Iran (ABRII), Iran

5Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

*Corresponding Author:
Mohammad Saeid Hejazi
Faculty of Pharmacy
Tabriz University of Medical Sciences
Tabriz, Iran
Tel: +98 (411) 337 2256
Fax: +98 (411) 334 4798
E-mail: [email protected],[email protected]
Mohammad Amin Hejazi
West & Northwest Agricultural Biotechnology Research Institute of Iran (ABRII)
Tabriz, Iran
Tel: +98 (411) 5156915-598
Fax: +98 (411) 3321615
Email: [email protected]

Published date: April 17, 2014; Published date: June 16, 2014; Published date: June 23, 2014

Citation: Hamidi M, Abdin MZ, Nazemyieh H, Hejazi MA, Hejazi MS (2014) Optimization of Total Carotenoid Production by Halorubrum sp. TBZ126 Using Response Surface Methodology. J Microb Biochem Technol 6:286-294. doi:10.4172/1948-5948.1000158

Copyright: © 2014 Hamidi M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

Carotenoids are one of the most diverse and broadly distributed classes of pigments in nature with a high number of biotechnological applications. Carotenoids have a broad range of functions, especially in relation to human health and their role as biological antioxidants. The increasing demand for consumption of natural carotenoids has raised interest in their bio-production. The objective of the present study was the analysis of environmental factors (temperature, pH and salinity) through response surface methodology (RSM) on the total carotenoid production of Halorubrum sp. TBZ126. In addition the effect of light was evaluated. Five levels of temperature, pH, and salinity were selected based on central composite design (CCD) and RSM to reach the optimum values for the cell growth and carotenoid production. Bio-production was carried out in an orbital shaker using a 10% (v/v) inoculum, and agitation at 120 rpm for 9 days in a non-illuminated environment. Dry cell weight was determined and total carotenoid was estimated by spectrophotometer. The production of biomass ranged from 0.04 to 0.84 g/l and the total carotenoid from 0.15 to 10.78 mg/l. The optimum conditions for cell growth and total carotenoid production in Halorubrum sp. TBZ126 cultures, were temperature 31ºC and 32ºC, pH 7.51 and 7.94 and NaCl (w/v) 18.33% and 20.55%, respectively. In conclusion, employing RSM design and under the light as an inducing factor, carotenoid production by Halorubrum sp. TBZ126 was elevated to about 145%. Additionally, TBZ126 could produce carotenoids at lower concentrations of NaCl (as low as 2.5%), in the absence of sodium acetate without elevating magnesium sulfate concentration.

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