Optimized Atrazine Degradation by Pleurotus ostreatus INCQS 40310: an Alternative for Impact Reduction of Herbicides Used in Sugarcane Crops
3Postgraduate Program in Biochemistry, Department of Biochemistry, Chemistry Institute, Federal University of Rio de Janeiro, Avenida Athos da Silveira Ramos, 149, Cidade Universitaria, Rio de Janeiro, RJ 21941-909, Brazil
- *Corresponding Author:
- Viridiana Santana Ferreira-Leitão
National Institute of Technology, Ministry of Science and Technology
Av. Venezuela 82, 302 Centro – CEP 20081-312 Rio de Janeiro – RJ – Brazil
Tel: +55 21-2123-1108/1109
Fax: +55 21-2123-1166
E-mail: [email protected]
Received date: April 03, 2013; Accepted date: May 03, 2013; Published date: May 06, 2013
Citation: Pereira PM, Sobral Teixeira RS, de Oliveira MAL, da Silva M, Ferreira- Leitão VS (2013) Optimized Atrazine Degradation by Pleurotus ostreatus INCQS 40310: an Alternative for Impact Reduction of Herbicides Used in Sugarcane Crops. J Microb Biochem Technol S12:006. doi: 10.4172/1948-5948.S12-006
Copyright: © 2013 Pereira PM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The herbicide atrazine (2-chloro-4-ethylamine-6-isopropylamine-s-triazine) is extensively used for weed control in sugarcane crops. The application of fungi for the biodegradation of xenobiotics has been studied with promising results. Therefore, atrazine degradation mediated by Pleurotus ostreatus INCQS 40310 was evaluated, and the involvement of ligninolytic enzymes along with the degradation process was also investigated. To promote high degradation percentages and rates, a fractional factorial experimental design was first used to determine the most significant medium components for atrazine degradation. This strategy improved atrazine degradation from 39.0% to 71.0% after 15 days, with the formation of different metabolites. Afterward, a 32 full factorial design was performed using the variables selected in the first part of this study. The salts FeSO4 and MnSO4 showed significant influence in the percentages and the rates of atrazine degradation. The medium optimization resulted in 90.3% and 94.5% of atrazine degradation after 10 days and 15 days, respectively. Although laccase activity was measured during the degradation process, it was not possible to correlate laccase activity with atrazine degradation. The results demonstrated the efficiency of P. ostreatus INCQS 40310 for atrazine degradation, thus demonstrating the potential of this fungus as a bioremediation agent.