Optimizing UHPLC Fittings and Connections: A Case Study
Eduard Rogatsky*, Shaynah B, Cai M and Daniel TS
Biomarker Analytical Resource Core, Einstein-Montefiore Institute for Clinical and Translational Research, Albert Einstein College of Medicine, USA
- *Corresponding Author:
- Eduard Rogatsky
Biomarker Analytical Resource Core
Einstein-Montefiore Institute for Clinical and Translational Research
Albert Einstein College of Medicine, USA
E-mail: [email protected]
Received Date: July 25, 2013; Accepted Date: August 16, 2013; Published Date: August 19, 2013
Citation: Rogatsky E, Shaynah B, Cai M, Daniel TS (2013) Optimizing UHPLC Fittings and Connections: A Case Study. J Chromatograph Separat Techniq 4:193. doi: 10.4172/2157-7064.1000193
Copyright: © 2013 Rogatsky E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We experienced persistent problems in achieving robust connections in UHPLC applications utilizing sub-3 micron particle size reversed phase chromatographic columns. Our application involved column testing, which involves frequent disconnection and re-connection of different columns into/from the flow path ofthe UHPLC system. In this paper we report our findings and recommendations.