Optimum Dietary Ca/P Ratio and Phytase for Growth and Bone Mineralization in Juvenile Clarias gariepinus Fed Soya Bean-Based Diet
- *Corresponding Author:
- Akpoilih BU
Department of Fisheries, University
of Port Harcourt, Faculty of Agriculture
PMB 5323, Port Harcourt, Nigeria
E-mail: [email protected]
Received Date: November 05, 2015; Accepted Date: November 30, 2015; Published Date: February 15, 2016
Citation: Akpoilih BU, Ajani EK, Omitoyin BO (2016) Optimum Dietary Ca/P Ratio and Phytase for Growth and Bone Mineralization in Juvenile Clarias gariepinus Fed Soya Bean-Based Diet. J Aquac Res Development 7:403. doi:10.4172/2155-9546.1000403
Copyright: © 2016 Akpoilih BU, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The research investigated the interactive effect of five phytase graded levels of 0 FTU/g (P0), 250 FTU/g (P1), 500 FTU/g (P2), 750 FTU/g (P3), 1000 FTU/g (P4) and four graded levels of roasted soya bean substitution at 25%, 50%, 75% and 100% in place of fish meal, respectively. All basal diets contained 0.20% calcium without added inorganic phosphorus, wheat and amino acids supplements so that the phosphorus, calcium, and Ca/P ratios of the five (5) were: S0 (1.31%, 1.85%, and 1.41), S1 (1.15%, 1.64%, and 1.43), S2 (1.01%, 1.28%, and 1.27), S3 (0.75%, 0.99%, and 1.30), and S4 (0.43%, 0.68%, and 1.59). 1092 fish of average weight 11.55 ± 0.2 g were randomly allocated to all experimental diet and fed 3% body for 84 days. There was no significant difference in final weight, weight gain and FCR S3P1 and S0P0 (P>0.05). Fish fed low phosphorus diet (100% soya bean) showed reduced total weight and weight gain compared to high (0%, 25%, 50%) and medium phosphorus diet (75%) with phytase addition (P<0.05). Fish fed phytase at 50-200 FTU/g and inorganic control had lower weight gain compared to S3P1 (P<0.05). Ca/P ratio correlated negatively with final weight (r=-0.439, P<0.01), weight gain (r=-0.431, P<0.01), SGR (r=-0.138, P>0.05), and PER (r=-0.210, P>0.05). Ca/P of S3P1 (1.41 ± 0.02) was not different from S0P0 (1.41 ± 0.01). Bone phosphorus, calcium and zinc were higher in 250 FTU/g compared to 0 FTU/g, 500 FTU/g, 750 FTU/g and 1000 FTU/g. In conclusion, Phytase improved growth performance (r=0.05) of juvenile Clarias gariepinus with optimum at 250 FTU/g; however, Ca/P affected performance (r=-0.431) and should range between 1.41-1.46 for optimum phytase activity.