Original Covalent Approach for Gold Nanorods Immobilization onto Acid-Terminated-Cysteamine Self-Assembled Monolayers for FT-SPR Biosensor Applications
- *Corresponding Author:
- Spadavecchia J
Laboratoire de Réactivité de Surface
UMR CNRS 7197
Université Pierre and Marie Curie–Paris VI
Site d’Ivry–Le Raphaël
94200 Ivry-sur-Seine, France
Tel : 33 4 37 42 35
E-mail: [email protected]
Received Date: May 05, 2015 Accepted Date: June 10, 2015 Published Date: June 20, 2015
Citation: Politi J, Stefano LD, Casale S, Spadavecchia J (2015) Original Covalent Approach for Gold Nanorods Immobilization onto Acid-Terminated-Cysteamine Self-Assembled Monolayers for FT-SPR Biosensor Applications. J Biosens Bioelectron 6:167. doi: 10.4172/2155-6210.1000167
Copyright: © 2015 Politi J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this paper, we report an original method to immobilize gold nanorods onto mixed self-assembled monolayers (SAMs) of Mercaptoundecanoic Acid (MUA), Mercaptohexanol (MOH) and cysteamine (CYS) onto planar gold surface. Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images revealed a remarkable shape and size narrow distribution on well functionalized gold surfaces, as well as a tendency to form linear assemblies after immobilization. The results highlight the good distribution of gold nanorods with an average length of 32.6 ± 0.9 nm and width of 13 ± 1.8 nm, the simplicity of the immobilization procedure of gold nanorods and the interest of using them as labels to enhance the sensitivity of FT-SPR-based sensors. gold nanostructured surface FT-SPR measurements of biorecognition using gold nanorods immobilized onto gold surfaces were performed at various prostatic antigen specific (PSA) concentrations, from 5 mg/L to 0.5 mg/L reaching a system sensitivity of 37 ± 2 cm-1/ mgL-1.