Osteoinduction and Antiosteoporotic Performance of Hybrid Biomaterial Chitosan-Bioactive Glass Graft: Effects on Bone RemodelingJebahi S1,2, Oudadesse H1*, Faouzi FZ1, Elleuch J2, Rebai T3, Keskes H3, Mostafa A1, El Feki A2 and El Feki H4
- *Corresponding Author:
- Oudadesse H
UMR-CNRS 6226, Campus de Beaulieu, Université de Rennes 1
263 Avenue du Général Leclerc, 35042 Rennes, France
E-mail: [email protected]
Received Date: August 07, 2013; Accepted Date: September 25, 2013; Published Date: September 30, 2013
Citation: Jebahi S, Oudadesse H, Faouzi FZ, Elleuch J, Rebai T, et al. (2013) Osteoinduction and Antiosteoporotic Performance of Hybrid Biomaterial Chitosan-Bioactive Glass Graft: Effects on Bone Remodeling. J Material Sci Eng 2:128. doi: 10.4172/2169-0022.1000128
Copyright: © 2013 Jebahi S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Osteoinductive and antiosteroporotic phenomena could be created by using synthetic biomaterials for applications in bone surgery.
In the present study, CH-based bioactive glass (BG-CH) with 17 wt% chitosan was elaborated by a freeze-drying process. BG-CH was implanted in the muscle and in the femoral condyles of ovariectomised rats. Grafted tissues were carefully removed for physico-chemical and histological analysis.
Several physic-chemical techniques (XRD, FT-IR, MEB, ICP-OES and NMR) were employed to highlight the effects of chitosan on the glass matrix before and after implantation.
The results of the study show that despite the non-additional osteogenic cells or agents, BG-CH is endowed with an osteoinductive property. After 8 weeks, 13C NMR spectra showed the characteristic signals of γ-carbons of the hydroxyproline (71 ppm) abundant in collagen.
γ-carboxyglutamate (55 ppm), which occurs in several other bone proteins like osteocalcin, indicating the BGCH degradation and the dominance of the bone tissue formation. Moreover, this study showed a rise in Ca and P ion concentrations in the implanted microenvironment, leading to the formation/deposition of Ca-P phases. Trace elements such as Zn and Fe were detected in the newly-formed bone and involved in the bone healing. The study highlights the suitability and the extensive applications of BG-CH composites and the clinically useful therapy in regenerative medicine.