Biochemistry & Analytical Biochemistry

Abstract

Overview of IgG-Reactivity in Therapeutic immunoglobulins Revealed by Protein Array Analysis

Sonia N

In addition to therapy for immune deficient patients, intravenous immune globulins (IVIg) are used to treat several autoimmune and inflammatory diseases. Several groups working to better understand how IVIg can modulate immune and inflammatory responses have proposed the involvement of self-reactive IgG present in IVIg. Consequently, a moreexhaustive characterization of IVIg repertoire could be very helpful for their better utilization. To increase our knowledge on IVIg repertoire, we have used the technology of protein array analysis to characterize a commercial IVIg preparation by screening its interaction with 9484 proteins of human origin. As a result, we have identified 67 proteins, which are strongly recognized by the IgG present in IVIg. Most of these targets are highlighted for the first time bringing new insight into IVIg mechanisms of action.