Palmitoleic Acid Infusion Alters Circulating Glucose and Insulin Levels
|Long NM, Burns TA, Volpi Lagreca G, Alende M and Duckett SK*|
|Department of Animal and Veterinary Sciences, Clemson University, Clemson, SC 29634, USA|
|Corresponding Author :||Susan K Duckett
Department of Animal and Veterinary Science
146 Poole Agricultural Center
Box 340311, Clemson, SC 29634-0311, USA
E-mail: [email protected]
|Received May 02, 2014; Accepted June 23, 2014; Published June 26, 2014|
|Citation: Long NM, Burns TA, Volpi Lagreca G, Alende M, Duckett SK (2014) Palmitoleic Acid Infusion Alters Circulating Glucose and Insulin Levels. J Metabolic Synd 3:148. doi:10.4172/2167-0943.1000148|
|Copyright: © 2014 Long NM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Objectives: The objectives of these studies are to evaluate: 1) uptake of a pulse dose of 13C16:1 cis-9 at varying levels into the blood and 2) glucose and insulin changes after pulse dose of 0 or 5 mg C16:1 cis-9/kg body weight (BW) under challenge conditions in obese lambs.
Methods: Two experiments were conducted to evaluate uptake of U-13C16:1 cis-9 into the blood and changes in glucose and insulin under challenges. In the first experiment, lambs (67.4 ± 1.4 kg BW, n=3) received jugular catheters and were used in a 3 x 3 Latin square. Treatments were 0, 2 or 5 mg/kg BW of U-13C16:1 cis-9 in 40% (wt/v) ethanol and blood samples were collected post infusion for glucose, fatty acid and insulin analyses. In the second experiment, lambs (86.7 ± 1.5 kg BW; n=4) received jugular catheters. Treatments were 0 or 5 mg/kg BW of C16:1 in 40% (wt/v) ethanol immediately followed by a glucose (0.25 g/kg) or insulin (0.02 mIU/kg) challenge.
Results: Both the 2 and 5 mg/kg BW dose of U-13C16:1 cis-9increased (P=0.003) C16:1 cis-9in serum compared to 0 mg/kg BW. The 5 mg/kg BW dose had a greater magnitude of increase for serum C16:1 and resulted in increased whole blood glucose levels for first 60 min and altered insulin levels for first 30 min. During the glucose tolerance test, C16:1bolus infusion increased (P=0.02) peak, overall, and area under the curve for plasma glucose levels. During the insulin challenge, C16:1-treated lambs had increased glucose levels (P=0.04) and peak, overall, and area under the curve plasma insulin (P=0.0001).
Conclusion: Palmitoleic acid infusion results in immediate uptake and clearance of serum palmitoleic acid, increases plasma glucose levels, and alters circulating insulin levels.