alexa Pap Test as the First Step in Screening Genetic Stabili
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

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Research Article

Pap Test as the First Step in Screening Genetic Stability in Cell-Based Therapy

Ana C. Irioda1, Larissa Zocche2, Carolina M. C. O. Souza3, Reginaldo J. Ferreira4, Eduardo Aliprandini5, Ricardo C. Cunha6, Julio C. Francisco7, Luiz C. Guarita-Souza8, Mariester Malvezzi9, Miriam P. Beltrame10, Lismary A. F. Mesquita11, Diogo Kuczera12, Juan C. Chachques13 and Katherine A. T. Carvalho14*

1MSC-student*. Isolation and Culture Proceedings. Cell Therapy and Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research, Pequeno Príncipe Faculty, Av. Silva Jardim 1632, Box 80.250-200, Curitiba, Paraná, Brazil Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Av. Coronel. Francisco Heráclito dos Santos 210, Box 81.531-970, Curitiba, Paraná, Brazil

2MSC-student*. Isolation, Culture and Differentiation Proceedings. Cell Therapy and Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research, Pequeno Príncipe Faculty, Av. Silva Jardim 1632, Box 80.250-200, Curitiba, Paraná, Brazil

3PhD-student*. Papanicolau Staining. Cell Therapy and Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research, Pequeno Príncipe Faculty, Av. Silva Jardim 1632, Box 80.250-200, Curitiba, Paraná, Brazil

4PhD- student. Literature Reviewer. Federal University of Technology, Cristo Rei 19, Box 85.002-490, Toledo, Paraná, Brazil

5PhD-student. Papanicolau Staining. Cell Therapy and Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research, Pequeno Príncipe Faculty, Av. Silva Jardim 1632, Box 80.250-200, Curitiba, Paraná, Brazil

6PhD- student. Literature Reviewer. Cell Therapy and Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research, Pequeno Príncipe Faculty, Av. Silva Jardim 1632, Box 80.250-200, Curitiba, Paraná, Brazil

7PhD- student. Papanicolau Staining. Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Av. Coronel. Francisco Heráclito dos Santos 210, Box 81.531-970, Curitiba, Paraná, Brazil

8Professor, MD and PhD. Discussion. Cardiovascular Surgery Department, Costantini Hospital, Rua Rosa Kaint Nadolny 190, Box 81.200-290, Curitiba, Paraná, Brazil

9Professor, MD, Citometric Analysis. Hospital de Clínicas, Haematology Department, Federal University of Paraná, Av. General Carneiro, 181 - Box 85.002-490, Curitiba, Paraná, Brazil

10PhD- student. Citometric Analysis. Hospital de Clínicas, Haematology Department, Federal University of Paraná, Av. General Carneiro, 181 - Box 85.002-490, Curitiba, Paraná, Brazil

11MD. Pathological Analysis. Pathology Department, Santa Casa de Misericórdia Hospital, Praça Rui Barbosa 694, Box 80.010-030, Curitiba, Paraná, Brazil

12PhD. Citometric Analysis. Cell Biology Department, Federal University of Paraná, Av. Coronel. Francisco Heráclito dos Santos 210, Box 81.531-970, Curitiba, Paraná, Brazil

13Professor, MD, PhD. Discussion. Laboratory of Biosurgical Research, Pompidou Hospital, University of Paris Descartes, Rue Leblanc, 20- Box 75015, Paris, France.

14Professor, MD and PhD. Experimental design, conclusion as writer of manuscript. Cell Therapy and Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research, Pequeno Príncipe Faculty, Av. Silva Jardim 1632, Box 80.250-200, Curitiba, Paraná, Brazil Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Av. Coronel. Francisco Heráclito dos Santos 210, Box 81.531-970, Curitiba, Paraná, Brazi

*Corresponding Author:
Katherine Athayde Teixeira de Carvalho, MD, PhD
The Pelé Pequeno Príncipe Institute
Child and Adolescent Health Research
Pequeno Príncipe Faculty, Av. Silva Jardim 1632
Box 80.250-200, Curitiba, Paraná, Brazil
Tel: 0055-41-3310-1719
Fax: 0055-41-3223-1689
E-mail: [email protected]

Received date: August 28, 2011; Accepted date: October 17, 2011; Published date: October 19, 2011

Citation: Irioda AC, Zocche L, Souza CMCO, Ferreira RJ, Aliprandini E, et al. (2011) Pap Test as the First Step in Screening Genetic Stability in Cell-Based Therapy. J Stem Cell Res Ther 1:106. doi:10.4172/2157-7633.1000106

Copyright: © 2011 Irioda AC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



 

Abstract

The possibility of cell modifications compromises the safety of stem cell therapy under standardized conditions. The aim of this study was to analyze adipose tissue-derived mesenchymal stem cells (AT-MSCs) using the Pap test as a first screening step to evaluate genetic stability. Methods: Human adipose tissue from six healthy female donors was obtained from elective liposuction procedures. The cells were isolated, cultivated at P2/P3, characterized by flow cytometric analysis, and differentiation induced. The AT-MSCs were stained by Papanicolaou (Pap) staining and analyzed according to the Bethesda classification, and viability-apoptosis relationships were evaluated. Results: The Pap test for Sample I indicated high-grade alterations consistent with genetic instability; for Samples II-V, atypical cells of undetermined significance; and for Sample VI, normal cells. Conclusions: These results demonstrate the potential of using the Pap test as an initial screening step to evaluate the genetic stability of cultured AT-MSCs as well for other adherent stem cells.

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