Parallel Effect of Nicotine and MK-801 on Brain Metabolism: An In vivo Non Invasive Near-Infrared Spectroscopy Analysis in Rats
Department of Biology, Neurosciences CEDD GlaxoSmithKline, Medicines Research Centre, Via A Fleming 4, 37135 Verona, Italy
- *Corresponding Author:
- Francesco Crespi
Department of Biology
Neurosciences CEDD GlaxoSmithKline
Medicines Research Centre
Via A. Fleming 4, 37135 Verona, Italy
Tel: 39 045 8218703
Fax: 39 045 8218196
E-mail: [email protected]
Received date: June 12, 2013; Accepted date: July 10, 2013; Published date: July 15, 2013
Citation: Crespi F (2013) Parallel Effect of Nicotine and MK-801 on Brain Metabolism: An In vivo Non Invasive Near-Infrared Spectroscopy Analysis in Rats. Curr Synthetic Sys Biol 1:101. doi: 10.4172/2332-0737.1000101
Copyright: © 2013 Crespi F. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Oxygenated haemoglobin, deoxyhaemoglobin and total blood volume are measured with our recently developed Near-Infrared Spectroscopy [NIRS] apparatus allowing in vivo non invasive real time monitoring of brain metabolism in anaesthetized rats. These measurements are indicative of the state of vascular activity and the state of the oxygen saturation, thus of the level of metabolism in the living tissue.
Nicotine is a natural alkaloid derived from tobacco that has been implicated in various effects ranging from addiction to toxic effects and neuro-protective actions. Here we analyse the influence of nicotine as well as that of the non competitive NMDA receptor antagonist MK-801 [(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d] cyclohepten-5,10-imine maleate)] upon brain metabolism in anaesthetized rats.
In the first 30min after treatment, nicotine decreases significantly although in a transient manner oxygenated haemoglobin and total blood volume while increasing significantly deoxyhaemoglobin. In addition, MK-801 performed in another group of rats was followed by changes in these three parameters that were similar to those monitored in nicotine treated rats.
The NIRS methodology appears to be apt to analyzing non-invasively and in real time the influence of systemic pharmacological treatments upon brain metabolism. In particular the data gathered show similarity of action of the two chemicals studied on influencing metabolic brain “behaviour” proposing that their central protective action may pass via the observed similar changes in brain metabolism. These changes could be a common mechanism of adaptation and protection towards neurotoxicity: mechanism that should be also considered in the intent of developing new pharmaceutical approaches for neuro-protective treatments.