PATTERN OF THE SENSITIVITY OF ALLIUM CEPA ROOT MERISTEM CELLS TO ENDOSRI, NUVAN AND KVISTIN PESTICIDES
|Aashiq H Kuchy1*, Aijaz A Wani2, Azra N Kamili1 and Bhat Mohd Skinder1
|Corresponding Author: Aashiq H Kuchy, Tel :+91 9596271756 E-mail: [email protected]|
|Received: 10th Aug-2015 Accepted: 10th Oct-2015|
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Cytotoxicity of two insecticides (Endosri & Nuvan) and one fungicide (Kvistin) was evaluated using Allium cepa (Onion) root inhibition test. Onion bulbs were exposed to different concentrations of Endosri (2000, 1000, 500, 250, 125, 60, 30, & 15ppm), Nuvan (400, 350, 300, 250, 200, 150, & 100ppm) and Kvistin (3000, 2000, 1000, 750, 500, 250, & 125ppm) for 5 days. Set of bulbs treated with distilled water (DW) was kept as the control. Effects on the growth and number of roots were recorded after the exposure period. EC50 values obtained for Endosri, Nuvan and Kvistin were 60, 200 and 500ppm, respectively. Both Endosri and Nuvan showed highly toxic effect and a significant (p≤0.05) decrease in both root length and number as compared to control. However, Kvistin was less toxic as compared to Endosri and Nuvan. Our results indicated that even very low concentrations of Endosri and Nuvan are highly toxic to Allium cepa root meristematic cells as compared to Kvistin fungicide. This toxicity could also contribute to several afflictions in other organisms after exposure to higher concentrations and a negative impact on the crop production as well.