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ISSN: 0974-7230

Journal of Computer Science & Systems Biology
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Review Article

PCR Primer Design: DREB Genes

Neha Garg1,3, Sachin Pundhir2, Anil Prakash3 and Anil Kumar2*

1Institute of Biochemistry & Biology, University of Potsdam, Karl- Liebknecht-STR 24-25,   Haus 20 D-14476,   Golm, Germany

2School of Biotechnology, Devi Ahilya University, Khandwa Rd., Indore-452001, India

3Biotechnology Department, Barkatullah University, Bhopal-462026, India

*Corresponding Author:
Dr. Anil Kumar
School of Biotechnology
Devi Ahilya University
Khandwa Rd., Indore-452001, India

Received Date: October 14, 2008; Accepted Date: December 20, 2008; Published Date: December 26, 2008

Citation: Garg N, Pundhir S, Prakash A., Kumar A (2008) PCR Primer Design: DREB Genes. J Comput Sci Syst Biol 1:021-040. doi: 10.4172/jcsb.1000002

Copyright: © 2008 Garg N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The polymerase chain reaction (PCR) is an enzymatic reaction which follows simple, predictable and well understood principles. Selective amplification of nucleic acid molecules, that are initially present in minute quantities, provides a powerful tool for analyzing nucleic acids. In this context, efficiency and sensitivity of the PCR largely depends on the efficiency of the primers that are employed for the amplification of a concerned gene. Environmental adversities like drought resulting in scarcity of water have detrimental effects on crop yields worldwide. Sustainable agricultural and food productivity requires development of stress resistant plant species like drought resistant crops that can with stand and flourish in scanty water level environments. A key to underlying such attempts is the molecular understanding of the discrete stress processes that are interwoven at multiple levels. In this review, we discuss about some of the contemporary developments in the area of stress resistance by plants along with the various approaches for the PCR primer designing of two key genes involved in drought resistance, DREB1A and DREB2A.

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