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PCR Typing of Mycobacterium ulcerans Lineages Based on Large Sequence Polymorphisms | OMICS International | Abstract
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
Open Access

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Research Article

PCR Typing of Mycobacterium ulcerans Lineages Based on Large Sequence Polymorphisms

Michael Kaser1,2*, Julia Hauser1,2 and Gerd Pluschke1,2

1Swiss Tropical and Public Health Institute, Switzerland

2University of Basel, Switzerland

*Corresponding Author:
Michael Kaser
Swiss Tropical and Public Health Institute
Socinstr. 57, 4002 Basel, Switzerland
Tel: +41-61-2848-365
Fax: +41-61-2848-101
E-mail: [email protected]

Received December 08, 2011; Accepted January 17, 2012; Published January 19, 2012

Citation: Kaser M, Hauser J, Pluschke G (2012) PCR Typing of Mycobacterium ulcerans Lineages Based on Large Sequence Polymorphisms . J Medical Microbiol Diagnosis S1:001. doi:10.4172/2161-0703.S1-001

Copyright: © 2012 Kaser M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Genetic fine typing of Mycobacterium ulcerans, the causative agent of the neglected tropical disease Buruli ulcer, has long been hampered by the remarkable genetic homogeneity of patient isolates. However, large sequence polymorphisms (LSPs), mainly originating from insertional-deletional genomic events, allow the differentiation between major lineages of M. ulcerans and other mycolactone producing mycobacteria (MPMs). Here, we describe an LSP-based rapid PCR typing scheme for these mycobacteria. Application of this approach to a selection of mycobacterial strains isolated from Buruli ulcer patients in Japan shows that they belong to the M. ulcerans Asian haplotype.

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