PCR-SBT Characterization of A New HLA Allele: A*02:548Valentina Cappuzzo*, Francesco Ingrassia, Rosalba Bavetta, Serena Mistretta, Maria Blando, Igea Vega, Emanuela Collura and Raimondo Marcenò
Laboratory of Tipizzazione Tessutale ed Immunogenetica, Medicina Trasfusionale – A.O, Ospedali Riuniti Villa Sofia-Cervello, via Trabucco, 180- 90146 Palermo, Italy
- *Corresponding Author:
- Valentina Cappuzzo
Laboratory of Tipizzazione Tessutale ed
Immunogenetica, Medicina Trasfusionale – A.O
Ospedali Riuniti Villa Sofia-
Cervello, via Trabucco
180- 90146 Palermo, Italy
E-mail: [email protected]
Received date: January 18, 2016; Accepted date: February 9, 2016; Published date: February 18, 2016
Citation: Cappuzzo V, Ingrassia F, Bavetta R, Mistretta S, Marceno R, et al. (2016) PCR-SBT Characterization of A New HLA Allele: A*02:548. Immunother Open Acc 2:112. doi:10.4172/2471-9552.1000112
Copyright: © 2016 Cappuzzo V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The human Major Histocompatibility Complex (MHC) lies within the short arm of chromosome 6 and is responsible for the production of Human Leukocyte Antigens (HLA). The HLA genes are the most polymorphic genes in the human genome, encoding over 13000 allelic variants. Our laboratories are involved in HLA tissue typing of patients, their relatives and volunteer donors for the Italian Bone Marrow Register. During HLA-A high resolution typing of our Caucasian bone marrow volunteer donors, we found a sequence largely homologous to A*02:01 with a mutation at position 367 a C instead of a T. This mutation results in a coding change from Tyrosine to Histidine in exon 3 (codon 99 TAT→CAT). This allele was then submitted to the WHO Nomenclature Committee via the IMGT/HLA Database for naming and was assigned the official name A*02:548.