Abstract

Biosensors are analytical devices composed of a recognition element of biological origin and a physiochemical transducer which converts the reaction into readable signals. The biosensors are providing their application in medical diagnosis and clinical analysis from last fifty years. In this work we have synthesized reduced graphene oxide, with this nano-film of RGO a FET- biosensor was developed and tested for the rapid detection of Rota-virus. It is a sensitive, specific and label free detection protocol. In the present methodology graphene; which is a single monomer unit of graphite was used and fabricated between the 3 μm apart micro-fabricated Au-electrode over hot plate at 70°C on a crystal surface by drop casting method. This thin film of graphene on FET crystal provide a better platform for attachment of antibodies through pyrene-NHS (Linker) complex. Pyrene- NHS is identified more suitable for antibody immobilization on the graphene film to be used in nano-immunosensor for the detection of specific antigens. The Rota-virus interation to its corresponding antibody were studied with respect to changes in the conductance in the graphene channel. This chemi-resistive FET transducer based 1D nanostructure have attracted much attention because of its rapid, sensitive, and cost effective point of care device for quantitative detection of Rota-virus so that gastroenteritis in infants can be detected early. The graphene nanofilm has been characterized by UV-Vis Spectroscopy, FT-IR, EDS, and SEM.

Keywords: Graphene oxide (GO); Reduced Graphene oxide (RGO); Field effect transistor (FET); Fourier transform infrared spectroscopy (FT-IR); Energy dispersive spectroscopy (EDS); Scanning electron microscopy (SEM)