Peripheral Blood B cell Subsets and BAFF/APRIL Levels and their Receptors are Disturbed in Rheumatoid Arthritis but not in Ankylosing Spondylitis
|Béatrice Gaugler1-3, Caroline Laheurte2,4, Ewa Bertolini5, Aurore Pugin4, Daniel Wendling5,6, Philippe Saas1-4*, Éric Toussirot4-7* on behalf of CBT-506|
|1INSERM UMR1098, Besançon, F-25020, France|
|2EFS Bourgogne Franche-Comté, UMR1098, Plateforme de BioMonitoring, F-25020 Besançon Cedex, France|
|3Université de Franche-Comté, UMR1098, Besançon, F-25020, France|
|4Centre Investigation Clinique Biothérapie INSERM CBT 506, CHRU, Besançon, France|
|5Service de Rhumatologie, CHRU, Besançon, France|
|6EA 4166 Agents Pathogènes et Inflammation Université de Franche Comté, Besançon, France|
|7Département Universitaire de Thérapeutique, Université de Franche Comté, Besançon, France|
|Corresponding Author :||Eric Toussirot
Professsor, Centre d’Investigation Clinique Biothérapie CBT-506
bâtiment St Joseph, CHRU Place St Jacques
25000 Besançon, France
Tel: 33 3 81 21 89 97
Fax: 33 3 81 21 85 22
E-mail: [email protected]
Professor, INSERM UMR1098
EFS Bourgogne Franche-Comté
1 Bd Fleming, 25000 Besançon, France
Tel: 33 3 81 615 615
Fax: 33 3 81 615 800
E-mail: [email protected]
|Received: August 13, 2013; Accepted: September 11, 2013; Published: September 18, 2013|
|Citation: Gaugler B, Laheurte C, Bertolini E, Pugin A, Wendling D, et al. (2013) Peripheral Blood B cell Subsets and BAFF/APRIL Levels and their Receptors are Disturbed in Rheumatoid Arthritis but not in Ankylosing Spondylitis. J Clin Cell Immunol 4:163. doi:10.4172/2155-9899.1000163|
|Copyright: © 2013 Gaugler B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Background: To evaluate the distribution of circulating B cell subsets and their expression of BAFF/APRIL receptors (BAFF-R, TACI and BCMA) as well as circulating levels of BAFF and APRIL in patients with rheumatoid arthritis (RA) or ankylosing spondylitis (AS) compared to healthy controls (HC).
Methods: 59 patients with RA, 61 patients with AS and 61 HC were evaluated. All patients were receiving traditional treatments and had not received prior biological treatment. Peripheral blood B cell subsets were assessed using multicolor flow cytometry using CD27, CD38 and IgD staining. Expression of BAFF-R, TACI and BCMA was analyzed on each cell subset.
Results: Distribution of peripheral B cells subsets was disturbed in RA compared to HC, with a decreased proportion of naïve and transitional B cells (p<0.005), whereas B cell subsets were comparable between AS and HC. Circulating BAFF did not differ between the three groups, while the ratio of BAFF/B cell number was significantly higher in RA compared to HC (p<0.001). Circulating APRIL levels were increased in RA compared to HC (p<0.001). Circulating BAFF and APRIL, and BAFF/B cell ratio did not differ between AS and HC. We also observed increased expression of BCMA, but not BAFF-R in RA, on both naïve and memory B cell subsets (post germinal center) (p<0.005), whereas TACI expression was decreased on memory B cells (p=0.001). The expression of BAFF/APRIL receptors did not differ between AS and HC.
Conclusion: Disturbances in B cell homeostasis in RA may promote B cell survival and deregulation, favoring the emergence of autoimmune B cells. Conversely, B cell homeostasis is not disrupted in AS.